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探讨采用高速液相色谱(HPLC)对各种卟啉衍生物的分离定量。使用装有HC—ODS Sil—x—1(2.6×250mm)色谱柱的液相色谱仪(Perkin—Flmer系列3),溶剂是10mM(毫摩尔浓度,下同)和5cM的磷酸钾缓冲液(前者pH6.0)流动相是含氢氧化四丁
To investigate the separation and quantification of various porphyrin derivatives by high performance liquid chromatography (HPLC). Using a liquid chromatograph (Perkin-Flmer Series 3) equipped with an HC-ODS Sil-x-1 (2.6 x 250 mm) column, the solvent was 10 mM (millimolar, the same applies) and 5 cM of potassium phosphate buffer The former pH6.0) mobile phase is tetrabutyl hydroxide