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探讨马尾松花粉硫酸酯化多糖对MIN6细胞胰岛素分泌和[Ca2+]i的作用。选取60%乙醇沉淀的马尾松花粉多糖(PPM60),氯磺酸-吡啶法得到硫酸酯化物(SPPM60);检测PPM60及SPPM60刺激后MIN6细胞胰岛素分泌量和[Ca2+]i的变化。观察钙离子抑制剂维拉帕米和低分子肝素的影响。PPM60对MIN6细胞胰岛素分泌几乎没有影响,SPPM60能显著促进胰岛素的分泌和[Ca2+]i升高(P<0.05)。维拉帕米和低分子肝素钠均可以降低SPPM60引起的胰岛素分泌增加,但差异不显著。同样两者均可显著性降低SPPM60引起的[Ca2+]i的升高(P<0.05)。维拉帕米可以明显抑制3.6 mg/mL葡萄糖的促胰岛素分泌(P<0.05)和升高[Ca2+]i的作用(P<0.01)。SPPM60可以单独促进胰岛素的分泌,其作用机制与葡萄糖刺激胰岛素分泌(GSIS)有一定相似性,但同时存在其他的信号通路。
To investigate the effect of pollen sulfated polysaccharides on insulin secretion and [Ca2 +] i in MIN6 cells. The 60% ethanol-precipitated masson pine pollen polysaccharide (PPM60) and chlorosulfonic acid-pyridine method were used to obtain the sulfated compounds (SPPM60). The changes of insulin secretion and [Ca2 +] i in MIN6 cells were detected by PPM60 and SPPM60. The effect of calcium ion inhibitor verapamil and low molecular weight heparin was observed. PPM60 had little effect on insulin secretion in MIN6 cells, and SPPM60 significantly increased insulin secretion and increased [Ca2 +] i (P <0.05). Both verapamil and LMWH could reduce the increase of insulin secretion induced by SPPM60, but the difference was not significant. Both of them also significantly reduced the increase of [Ca2 +] i induced by SPPM60 (P <0.05). Verapamil significantly inhibited insulin-induced insulin secretion (P <0.05) and increased [Ca2 +] i release of 3.6 mg / mL glucose (P <0.01). SPPM60 can promote the secretion of insulin alone, its mechanism of action and glucose-stimulated insulin secretion (GSIS) have some similarities, but there are other signaling pathways.