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目的:检测模拟失重48 h人肺微血管内皮细胞(HMVEC-L)miR-1246和miR-1261表达的改变,利用生物信息学方法分析预测其靶基因。方法:以回转器行模拟失重效应48 h的HMVEC-L为模拟失重组,同时设正常重力对照组,比较两组细胞miRNA表达谱的变化,应用Real-time PCR检测HMVEC-L细胞miR-1246和miR-1261的表达,通过Target Scan、miRDB和miRanda对miR-1246和miR-1261的靶基因进行预测以及功能注释和通路富集分析。结果:与正常重力对照组比较,模拟失重48 h可引起HMVEC-L细胞中29种miRNA呈现显著差异性表达,miR-1246和miR-1261显著降低,差异有统计学意义(P<0.05);生物信息学分析显示miR-1246和miR-1261的靶基因涉及生物过程、分子功能及细胞组成等多种功能,主要参与了磷酸化通路、凋亡相关通路和细胞骨架等相关分子的调控。结论:模拟失重48 h后可引起HMVEC-L细胞miR-1246和miR-1261的表达降低,提示其可能参与了模拟失重后内皮细胞功能异常的调控。
OBJECTIVE: To detect the changes of miR-1246 and miR-1261 expression in human pulmonary microvascular endothelial cells (HMVEC-L) 48 h after simulated weightlessness, and to predict their target genes by using bioinformatics methods. Methods: The HMVEC-L model of simulated weightlessness was simulated by weight loss at 48-hour rotator in simulated weightlessness group. At the same time, normal gravity control group was used to compare the expression of miRNA in two groups. Real-time PCR was used to detect the expression of miR-1246 And miR-1261, target genes of miR-1246 and miR-1261 were predicted by Target Scan, miRDB and miRanda as well as functional annotation and pathway enrichment analysis. Results: Compared with the normal control group, 48 miRNAs in HMVEC-L cells were significantly differentially expressed after simulated weightlessness for 48 h, while miR-1246 and miR-1261 were significantly decreased (P <0.05). Bioinformatics analysis showed that the target genes of miR-1246 and miR-1261 involved in many functions such as biological processes, molecular functions and cellular components, and were mainly involved in the regulation of phosphorylation pathway, apoptosis-related pathways and cytoskeleton related molecules. Conclusion: The expression of miR-1246 and miR-1261 in HMVEC-L cells decreased after 48-hour simulated weight loss, suggesting that they may be involved in the regulation of dysfunction of endothelial cells after simulated weightlessness.