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讨论了共焦扫描光学显微镜的高分辨率性质,指出共焦扫描显微镜由于采用点探测器,因而视场大大减小,信噪比大大提高,同时每幅图像逐点扫描形成,在光学系统信息能力不变的前提下,系统的空间域通带宽度增加和时域通带宽度减小。因而可成高分辨率的像,特别是其独特的深度分辨率特性使得可以实现光学断层扫描成像。给出了所研制的共焦扫描荧光显微镜所获得光学断层扫描图像
The high-resolution nature of the confocal scanning optical microscope is discussed. Since the confocal scanning microscope uses a point detector, the field of view is greatly reduced and the signal-to-noise ratio is greatly increased. Meanwhile, each image is scanned point by point. In the optical system information Under the premise of the same capability, the increase of the system bandwidth in the spatial domain and the decrease of the passband in the time domain. Thus, high-resolution images, especially their unique depth resolution properties, enable optical tomography imaging. The optical tomography images acquired by confocal scanning fluorescence microscope were given