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系统研究了在表面活性剂ＴｒｉｔｏｎＸ一１００存在下，新色原３，５一ｄｉＢｒ一ＰＡＤＡＰ与Ｚｎ（Ⅱ）的显色反应性能，建立了一种快速、灵敏、准确测定血清锌的比色法。用本法作血清锌测定，锌含量在０～４μｍｏｌ·Ｌ￣（－１）范围内线性良好，表现摩尔吸收系数为１．１２×１０５Ｌ·ｍｏｌ￣（－１）·ｃｍ￣（－１）。批内ＣＶ为２．８％、批间ＣＶ为３．４％，平均回收率９９．３％。本法（Ｙ）与原子吸收分光光度法（Ｘ）比较，两法差异无显著性意义（Ｙ＝１．００５Ｘ一０．０１２，Ｙ为０．９９３７，Ｐ＞０．０５）。
The color reaction of new chromogen 3,5-diBr-PADAP and Zn (Ⅱ) in the presence of surfactant Triton X-100 was studied systematically. A rapid, sensitive and accurate colorimetric method for the determination of serum zinc . The determination of serum zinc by this method showed a good linearity in the range of 0 ~ 4μmol·L ~ (-1), indicating a molar absorption coefficient of 1.12 × 105L · mol ~ (-1) · cm ~ (-1) . The intra-assay CV was 2.8%, the intra-assay CV was 3.4%, and the average recovery was 99.3%. There was no significant difference between the two methods (Y = 1.005X-0.012, Y = 0.9937, P> 0.05) by comparing this method with atomic absorption spectrophotometry (X).