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目的:探讨参附益心颗粒对心衰大鼠心肌c-fos,c-myc表达的影响。方法:随机将150只雄性SD大鼠分为造模组130只,假手术组20只。造模组结扎左冠状动脉前降支,假手术组手术方法同上,仅在冠状动脉前降支下穿线不结扎。造模8周后,经超声测定大鼠左室射血分数(left ventricular ejection fraction,LVEF)<50%者随机分为5组(模型组、卡托普利组、氯沙坦组、参附益心颗粒(5.48,21.90 g.kg-1.d-1)2个剂量组,每组10只,另选假手术组大鼠10只。假手术组及模型组以纯净水ig,药物干预组将药物溶于纯净水中ig给药,每天1次,疗程4周。4周后超声测定LVEF,称重后,处死测左室重量指数(left ventricular mass index,LVMI),免疫组化法检测大鼠心肌中c-fos,c-myc表达。结果:21.90 g.kg-1.d-1参附益心颗粒可显著提高心衰大鼠LVEF,降低LVMI,抑制心衰大鼠心肌中c-fos,c-myc表达。结论:参附益心颗粒能够通过抑制心衰大鼠心肌中c-fos,c-myc表达,抑制心肌重塑。
Objective: To investigate the effect of Shenfu Yixin Granule on the expression of c-fos and c-myc in myocardium of rats with heart failure. Methods: 150 male SD rats were randomly divided into modeling group 130, sham group 20. Ligation of the anterior descending branch of the left anterior descending artery was performed in the model group. The surgical method in the sham operation group was the same as above, and the ligament was not ligated only in the anterior descending coronary artery. After 8 weeks of modeling, rats with left ventricular ejection fraction (LVEF) <50% were randomly divided into 5 groups (model group, captopril group, losartan group, Yixin Granule (5.48,21.90 g.kg-1.d-1) 2 dose groups, 10 rats in each group and 10 rats in sham-operation group.The rats in sham operation group and model group were treated with pure water ig and drug intervention The drug was dissolved in pure water ig administration once a day for 4 weeks.After 4 weeks, the LVEF was measured by ultrasound and after weighing, the left ventricular mass index (LVMI) The expression of c-fos and c-myc in the myocardium of rats were observed.Results: 21.90 g.kg-1.d-1 Shenfuyixin Granule could significantly improve the LVEF, LVMI, -fos and c-myc.Conclusion: Shen-Fu-Yi-Xin Granule can inhibit myocardial remodeling by inhibiting the expression of c-fos and c-myc in cardiac muscle of heart failure rats.