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目的探讨OX40L基因变异与早发急性冠状动脉综合征风险之间的关系。方法采用聚合酶链反应-序列特异性扩增(PCR-SSP)技术检测187例年龄≤55岁、早发急性冠状动脉综合征(ACS)患者和138例冠状动脉造影正常者rs3850641位点A/G变异的基因型、等位基因及其分布频率;采用冠状动脉造影测量病变血管支数(DVN)和狭窄程度积分(SSI);应用ELISA法测试血浆sOX40L、血管细胞黏附分子(VCAM-1);应用散射比浊法测试血浆C反应蛋白(CRP)水平;分析OX40L基因变异与ACS风险、冠状动脉病变程度和血浆3个细胞因子之间的关系。结果OX40L基因A/G变异与早发ACS风险显著关联,G等位基因比野生型早发ACS风险增高2.031倍(OR=2.03),变异型比野生型DVN、SSI、血浆OX40L、VCAM-1和CRP显著增高。结论OX40L基因变异可能与早发ACS风险和冠状动脉病变程度有关;OX40L过度表达、细胞黏附和炎症反应可能是ACS发病的分子生物学基础。
Objective To investigate the relationship between OX40L gene mutation and the risk of early-onset acute coronary syndrome (ACS). Methods A total of 187 patients aged ≤55 years with early onset acute coronary syndrome (ACS) and 138 patients with normal coronary artery angiography were analyzed by polymerase chain reaction-sequence specific amplification (PCR-SSP) G variant genotypes, alleles and frequency of distribution; using coronary angiography to measure lesion vessel count (DVN) and stenosis degree (SSI); using ELISA test plasma sOX40L, vascular cell adhesion molecule (VCAM-1) ; The level of plasma C-reactive protein (CRP) was measured by nephelometry; the relationship between OX40L gene mutation and ACS risk, the degree of coronary artery lesion and plasma 3 cytokines were analyzed. Results The A / G mutation of OX40L gene was significantly associated with the risk of early onset ACS. The G allele was 2.031 fold (OR = 2.03) higher than that of wild type premature ACS patients. The variant genotype was significantly higher than those of wild type DVN, SSI, plasma OX40L, VCAM- And CRP increased significantly. Conclusion OX40L gene mutation may be related to the risk of premature coronary artery disease and coronary artery disease. OX40L overexpression, cell adhesion and inflammatory reaction may be the molecular basis of the pathogenesis of ACS.