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目的 建立葛根芩连汤有效部位中黄芩苷和葛根素的RP HPLC含量测定方法。方法 采用YWG C18色谱柱 (2 5 0mm× 4 6mm ,10 μm) ,黄芩苷含量测定流动相为甲醇 -水 -磷酸 (47∶ 5 3∶0 2 ) ,流速 :1 0mL/min ,检测波长 :2 80nm ;葛根素含量测定流动相为甲醇 -水 (2 5∶ 75 ) ,流速 :1 0mL/min ,检测波长 :2 5 0nm。结果 黄芩苷平均回收率为 98 61% ,RSD =2 77% (n =5 ) ;葛根素平均回收率为 10 2 4 % ,RSD =2 70 % (n =5 )。结论 所建立的方法简便、准确 ,可作为葛根芩连汤有效部位的质量控制方法
Objective To establish a method for the determination of baicalin and puerarin in the effective fraction of Gegen Qilian Decoction by RP HPLC. Methods A YWG C18 column (250 mm×4 6 mm, 10 μm) was used. The baicalin was determined to be methanol-water-phosphoric acid (47: 5 3∶0 2), the flow rate was 10 mL/min, and the detection wavelength was: 2 80nm; Determination of puerarin content The mobile phase was methanol-water (25: 75), flow rate: 10 mL/min, detection wavelength: 250 nm. Results The average recovery rate of baicalin was 98 61%, RSD =2 77% (n =5), the average recovery of puerarin was 10 2 4 %, and RSD =2 70% (n =5). Conclusion The established method is simple and accurate. It can be used as a quality control method for the effective part of Pueraria sinensis decoction.