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色谱法测定乙苯和二甲苯时,各组分色谱峰相互重叠而影响测定结果。本文用高效液相色谱联用二极管阵列检测器(HPLC-DAD)测定乙苯和二甲苯,采集7．20-8．53min,205-235nm范围内的二维色谱数据,再用直观推导式演进特征投影法(HELP)和交替最小二乘法(ALS)计算,依靠乙苯、二甲苯各异构体之间紫外吸收光谱的微小差异,解析它们的重叠色谱峰。各组分浓度在1-50μg/mL,浓度比为1∶10范围内的分辨结果令人满意,分辨下限为0．2μg/mL,该法成功地用于大气环境中乙苯和二甲苯异构体的测定。结果表明：HELP结合ALS适用于解析色谱严重重叠的多组分体系。
When chromatographic determination of ethylbenzene and xylene, the chromatographic peaks of each component overlap each other and affect the determination results. In this paper, high performance liquid chromatography with diode array detector (HPLC-DAD) determination of ethylbenzene and xylene, collecting 7.20-8.53min, 205-235nm two-dimensional chromatographic data, and then intuitively derived evolution Characteristic projection method (HELP) and alternating least squares (ALS) calculations rely on the small differences between the UV absorption spectra of the isomers of ethylbenzene and xylene to resolve their overlapping peaks. The resolution of each component in the range of 1-50μg / mL and the concentration ratio of 1:10 is satisfactory, with the lower limit of detection being 0.2μg / mL. The method has been successfully applied to the determination of ethylbenzene and xylene in the atmosphere Determination of the structure. The results show that HELP combined with ALS is suitable for the analysis of multicomponent systems with severe chromatographic overlap.