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目的：观察活动期和静止期狼疮肾炎（ＬＮ）外周血单个核细胞（ＰＢＭＣ）Ｂ７－１（ＣＤ８０）和 Ｂ７－２（ＣＤ８６）ｍＲＮＡ表达及其与疾病活动的关系，以探讨 Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达异常在 ＬＮ自身免疫发生中的作用及临床意义。方法：以正常人为对照，分离１５例活动期和１３例静止期的ＬＮ患者ＰＢＭＣ，采用半定量反转录ＰＣＲ的方法，检测ＰＢ－ＭＣ的 Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达。Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达与 ＳＬＥ疾病活动评分指数（ＳＬＥＤＡＩ）的相关关系用直线相关分析。结果：静止期 ＬＮ组 Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达与正常人对照组相比无显著性差异（Ｐ＞０．０５），活动期ＬＮ组Ｂ７－１ｍＲＮＡ有明显的上升（Ｐ＜０．０５），Ｂ７－２ ｍＲＮＡ表达增高更加显著（Ｐ＜ｏ．０１）。Ｂ７－２ ｍＲＮＡ的增高与ＳＬＥＤＡＩ呈直线正相关关系（ｒ＝０．８６４１，Ｐ＜０.０５）。结论：活动期狼疮性肾炎存在着Ｂ７ｍＲＮＡ表达的异常，Ｂ７－２ｍＲＮＡ的增高与 ＳＬＥＤＡＩ呈直线正相关关系。
Objective: To observe the expression of B7-1 (CD80) and B7-2 (CD86) mRNA in peripheral blood mononuclear cells (PBMCs) of active and quiescent lupus nephritis (LN) and their relationship with disease activity, Abnormal expression of B7-2 mRNA in LN autoimmunity and its clinical significance. Methods: PBMC from 15 patients with LN and 13 patients with quiescent LN were isolated from healthy volunteers. The expression of B7-1 and B7-2 mRNA in PB-MC was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Correlation between B7-1 and B7-2 mRNA expression and SLE disease activity score index (SLEDAI) was analyzed by linear correlation analysis. Results: The expression of B7-1 mRNA and B7-2 mRNA in quiescent LN group was not significantly different from that in normal control group (P> 0.05). The activity of B7-1 mRNA in active LN group was significantly increased (P <0. 05), B7-2 mRNA expression increased more significantly (P <0.01). There was a linear positive correlation between B7-2 mRNA and SLEDAI (r = 0.8641, P <0.05). Conclusion: There is abnormal expression of B7mRNA in active lupus nephritis. The increase of B7-2mRNA is positively correlated with SLEDAI.