文献报道,研究62种韩国植物对HIV-1蛋白酶的抑制作用,其中山楂(Crataegus pinnatifida)叶有显著的抑制活性。作者经研究报道,山楂叶(3.5kg)在室温下用甲醇提取3次,真空回收溶剂,得到的干燥提取物混悬于水,依次用二氯甲烷、乙酸乙酯和正丁醇提取,二氯甲烷部分经硅胶柱分离,得到了抑制HIV-1蛋白酶的活性 Reported in the literature, 62 species of Korean plants were inhibited against HIV-1 protease, among which Crataegus pinnatifida had significant inhibitory activity. The authors reported that hawthorn leaves (3.5kg) were extracted with methanol three times at room temperature and the solvents were recovered in vacuo. The resulting dried extracts were suspended in water and extracted sequentially with dichloromethane, ethyl acetate and n-butanol. The methane fraction was separated on a silica gel column to obtain the activity of inhibiting HIV-1 protease