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原发性阿米巴性脑膜脑炎(PAM)是新近认识的由自由生活阿米巴感染所致的疾病。本文报道实验性PAM鼠脑中γ-氨基丁酸(GABA)的代谢改变。实验用体重10～12g瑞士株小鼠,每只鼻内接种纯培养的致病株柯氏棘阿米巴1.4×10~4个,接种后4天,出现严重的PAM病理症状,即将实验鼠以及对照鼠断颅处死,取出脑组织保存于冷盐水中。用冰冷蒸馏水制备成10％的全脑组织匀浆。为进行不同区域脑组织的研究,将脑组织分成前脑(嗅叶与
Primary amoebic meningoencephalitis (PAM) is a newly recognized disease caused by free-living amoebic infections. This article reports the metabolic changes of γ-aminobutyric acid (GABA) in the brain of experimental PAM rats. The experimental mice weighing 10-12 g were inoculated intranasally with purely cultured pathogenic strains of Acanthamoeba armigera 1.4 × 10 ~ 4, 4 days after inoculation, serious symptoms of PAM pathology, the experimental mice As well as the control mice died of craniocerebral removal of brain tissue stored in cold saline. Prepare 10% whole brain homogenate with ice-cold distilled water. For different regions of the brain tissue research, the brain tissue into the forebrain (olfactory leaf and leaves