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目的:观察莪术醇对人结直肠癌Lo Vo细胞裸鼠移植瘤的抑制作用,探讨其可能的机制。方法:建立人结直肠癌Lo Vo细胞裸鼠移植瘤模型,成瘤后将其随机分为莪术醇高、中、低剂量(80,40,20 mg·kg-1)组,模型组,空白组,每天ig给药1次,给药21 d。用逆转录聚合酶链反应(RT-PCR)方法和免疫组化方法(IHC)检测瘤组织中血管生成因子(VEGF)、环氧合酶-2(COX-2)的表达。结果:与模型组比较,莪术醇高、中、低剂量组能明显降低肿瘤的瘤体积、瘤重及VEGF,COX-2的表达,均呈剂量依赖性。莪术醇高、中、低剂量组抑瘤率分别为66.88%,42.08%,25.73%;模型组VEGF表达量与莪术醇各组比较,差异有统计学意义(P<0.05);模型组COX-2表达量与莪术醇中、高剂量组比较,差异具有统计学意义(P<0.05),VEGF和COX-2表达具有明显相关性(r=0.874,P<0.01)。结论:莪术醇抑制结直肠癌Lo Vo细胞裸鼠移植瘤生长的机制可能与下调VEGF和COX-2表达有关,并且可能通过COX-2/前列腺素E2(PGE2)/VEGF信号通路发挥抑制结直肠癌生长的作用。
Objective: To observe the inhibitory effect of Curcumol on human colorectal carcinoma LoVo cell xenografts in nude mice and to explore its possible mechanism. Methods: Human colorectal cancer LoV cell xenografts were established in nude mice. After tumorigenesis, they were randomly divided into curcumol high, medium and low dose groups (80, 40, and 20 mg · kg -1) Group, once daily ig administration, administration 21 d. The expression of VEGF and COX-2 in tumor tissue was detected by RT-PCR and IHC. Results: Compared with the model group, Curcumol high, medium and low dose groups can significantly reduce the tumor volume, tumor weight and VEGF, COX-2 expression in a dose-dependent manner. The tumor inhibition rates of curcumol in high, medium and low dose groups were 66.88%, 42.08% and 25.73%, respectively. The expression of VEGF in model group was significantly different from that in curcumol group (P <0.05) (P <0.05). There was a significant correlation between the expression of VEGF and COX-2 (r = 0.874, P <0.01). CONCLUSION: Curcumol inhibits the growth of xenografts in nude mice with colorectal cancer LoV cells may be related to the down-regulation of the expression of VEGF and COX-2, and may inhibit the growth of colorectal cancer through the COX-2 / PGE2 / VEGF signaling pathway The role of cancer growth.