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目的研究Pitx2基因在斑马鱼牙齿早期发育阶段的表达。方法利用RT-PCR技术直接克隆Pitx2特异性基因片段,制作针对Pitx2的基因探针,选取斑马鱼发育早期多个时段的胚胎进行整胚原位杂交。结果成功克隆Pitx2基因,合成针对Pitx2的基因探针,获得Pitx2基因在斑马鱼早期发育阶段的表达情况。结论了解Pitx2基因在斑马鱼早期发育阶段中的表达情况。
Objective To study the expression of Pitx2 gene in the early development of zebrafish teeth. Methods The gene fragment of Pitx2 was directly cloned by RT-PCR and the gene probe for Pitx2 was made. Embryos of multiple stages of early zebrafish development were selected for in situ hybridization. Results The Pitx2 gene was successfully cloned and the gene probe targeting Pitx2 was synthesized. The expression of Pitx2 gene in the early stage of zebrafish development was obtained. Conclusion The expression of Pitx2 gene in early stage of zebrafish development was studied.