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目的探讨西达本胺联合地西他滨对霍奇金淋巴瘤(HL)细胞株Hs445和L428细胞增殖及凋亡的影响。方法采用MTT法检测西达本胺单用或联合使用地西他滨对Hs445和L428细胞的增殖与抑制作用,流式细胞术检测细胞凋亡情况及线粒体膜电位变化,Western blot检测细胞内cleaved PARP蛋白的表达。结果西达本胺作用24、48、72 h可抑制Hs445和L428细胞增殖,且呈现浓度和时间依赖性,对2种细胞的IC50(μmol/L)分别为1.91和3.46。西达本胺(μmol/L)(对Hs445为0.25、0.5,对L428为1、2)与地西他滨(μmol/L)(0.5、2)分别联合作用72h,对2种细胞的增殖抑制率(%)分别为35.76±0.97、54.20±1.32、54.12±1.24、73.13±0.45,41.05±0.67、56.40±0.39、67.80±0.89、82.35±1.45,对应浓度的西达苯胺单药组的增殖抑制率(%)为12.02±1.41、31.00±0.90,18.03±0.91、36.00±0.21(P<0.05),且均为两药相互作用的协同指数(CI)<1。0.5μmol/L西达本胺单用或其与2μmol/L地西他滨联合作用48 h后Hs445细胞凋亡率(%)分别为13.22±3.12 vs 67.26±7.87(P<0.01);2μmol/L西达本胺单用或其与2μmol/L地西他滨联合作用48 h后L428细胞凋亡率(%)分别为34.03±4.67 vs 70.58±5.76(P<0.01);联合用药组cleaved PRAP蛋白表达和线粒体膜电位的下降也明显高于单药组。结论西达本胺可抑制HL细胞株增殖并诱导其凋亡,与地西他滨联用对HL细胞株的增殖抑制及诱导凋亡具有明显著的协同效应。
Objective To investigate the effects of cialylamine combined with decitabine on the proliferation and apoptosis of Hodgkin lymphoma (HL) cell lines Hs445 and L428. Methods The proliferation and inhibition of Hs445 and L428 cells treated with citalophamine alone or in combination with cediamine were detected by MTT assay. The apoptosis and mitochondrial membrane potential were detected by flow cytometry. The cleaved PARP protein expression. RESULTS: Cisamine inhibited the proliferation of Hs445 and L428 cells in 24 h, 48 h and 72 h, both in a concentration- and time-dependent manner. The IC50 (μmol / L) of the two cells were 1.91 and 3.46, respectively. The combined effect of daunorubicin (μmol / L) (0.25, 0.5 for Hs445, 1, 2 for L428) and dx (0.5, 2) The inhibition rates (%) were 35.76 ± 0.97, 54.20 ± 1.32, 54.12 ± 1.24, 73.13 ± 0.45, 41.05 ± 0.67, 56.40 ± 0.39, 67.80 ± 0.89, 82.35 ± 1.45, respectively The inhibition rates (%) were 12.02 ± 1.41, 31.00 ± 0.90, 18.03 ± 0.91 and 36.00 ± 0.21 (P <0.05), and the synergistic index (CI) The apoptotic rate (%) of Hs445 cells treated with amoxicillin alone or combined with 2μmol / L decitabine for 48 h were 13.22 ± 3.12 vs 67.26 ± 7.87 (P <0.01), respectively. The apoptosis rate of Hs445 cells treated with 2μmol / (48.03 ± 4.67 vs 70.58 ± 5.76, P <0.01). The cleaved PRAP protein expression and mitochondrial membrane potential Decline was also significantly higher than the single drug group. CONCLUSION: Cedicillamine can inhibit the proliferation and induce the apoptosis of HL cell line, and it has obvious synergistic effect with decitabine on the proliferation inhibition and apoptosis induction of HL cell line.