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目的:探讨中药神经生长液对神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)模型鼠的治疗作用。方法:7周龄雄性C57BL/6小鼠80只,随机分为阴性组、模型组、美多芭组、中药组,每组20只。运用腹腔注射MPTP制备PD模型,通过灌胃的方法对模型进行中药神经生长液和美多芭治疗,以0.9%氯化钠溶液为阴性对照。通过记录体质量,检测转棒实验和旷场实验行为学变化;用酪氨酸羟化酶(TH)的免疫染色中脑黑质多巴胺能神经元;通过HPLC检测纹状体多巴胺(DA)含量。结果:(1)制模后,模型动物体质量较阴性组降低,差异无统计学意义。灌胃后小鼠的体质量增加,各组之间仍无差异。(2)行为学检测显示,模型动物在行为学检测上较阴性组均明显降低(P<0.01)。灌胃后中药组和美多芭组在行为学检测上比模型组都明显好转(P<0.01,P<0.05)。(3)免疫组化结果显示,灌胃后中药组TH阳性细胞数比模型组和美多芭组明显增加(P<0.01,P<0.05)。(4)HPLC结果显示,灌胃后模型组DA含量减少(P<0.05),美多芭组和中药组DA含量有增加趋势,但差异无统计学意义。结论:中药神经生长液对MPTP诱导的PD模型鼠有一定的治疗作用。
Objective: To investigate the therapeutic effect of traditional Chinese medicine nerve growth fluid on Parkinson’s disease (PD) model rats induced by neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Methods: Eighty male C57BL / 6 mice, aged 7 weeks, were randomly divided into a negative group, a model group, a metoprolol group and a Chinese herb group, with 20 rats in each group. The PD model was made by intraperitoneal injection of MPTP. The model was treated with traditional Chinese medicine nerve growth solution and metoprolol by intragastric administration, and 0.9% sodium chloride solution was taken as negative control. By recording the body weight, the behavior of the rods and the open-field behavioral changes were detected. The dopaminergic neurons in the substantia nigra were stained with tyrosine hydroxylase (TH). The contents of dopamine (DA) . Results: (1) After modeling, the body weight of the model animals was lower than that of the negative group, the difference was not statistically significant. After gavage, the body weight of mice increased, there was no difference between the groups. (2) Behavior tests showed that the model animals in the behavioral tests were significantly lower than the negative group (P <0.01). After administration of gavage, the Chinese medicine group and the metopil group showed significantly better behavioral tests than the model group (P <0.01, P <0.05). (3) The results of immunohistochemistry showed that the number of TH-positive cells in the TCM group increased significantly (P <0.01, P <0.05) than that in the model group and the metoprol group. (4) The results of HPLC showed that the content of DA decreased in the model group after intragastric administration (P <0.05), and the content of DA in the two groups had an increasing trend, but the difference was not statistically significant. Conclusion: The traditional Chinese medicine nerve growth fluid has a certain therapeutic effect on MPTP-induced PD model rats.