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【目的】应用超高效液相色谱配以串联质谱技术和二极管阵列检测技术对蓝、紫粒小麦籽粒中的花色苷进行分离与鉴定,揭示蓝、紫粒小麦籽粒花色苷组成成分。【方法】小麦籽粒花色苷用90%甲醇水溶液(含0.5%甲酸)超声波提取,SPEC18柱净化处理。应用超高效液相色谱串联质谱对蓝、紫粒小麦籽粒中的花色苷提取物进行母离子扫描,初步确定蓝、紫粒小麦籽粒中的花色苷种类,用全扫描、子离子扫描,多反应检测技术对蓝、紫粒小麦籽粒中的花色苷组分和含量进行分析。【结果】蓝、紫粒小麦籽粒中含有14种不同种类的花色苷类化合物,且不同的蓝、紫粒小麦籽粒中花色苷的种类与含量不同。【结论】明确了蓝、紫粒小麦籽粒中花色苷的组分与含量,建立了应用质谱快速分离与鉴定蓝、紫粒小麦籽粒中花色苷的方法。
【Objective】 The separation and identification of anthocyanins in blue and purple wheat grains by ultra performance liquid chromatography coupled with tandem mass spectrometry and diode array detection revealed the composition of anthocyanins in blue and purple wheat grains. 【Method】 The anthocyanins in wheat grain were extracted with 90% aqueous methanol solution (containing 0.5% formic acid) and cleaned up by SPEC18 column. Application of ultra performance liquid chromatography-tandem mass spectrometry for precursor ion scanning of anthocyanin extracts from blue and purple wheat grains to determine anthocyanin species in blue and purple wheat grains, using full scan, ion scan, multiple reaction Detection technology of blue and purple grain of anthocyanin composition and content analysis. 【Result】 The results showed that there were 14 different kinds of anthocyanins in blue and purple wheat grains, and the types and contents of anthocyanins in different blue and purple wheat grains were different. 【Conclusion】 The composition and content of anthocyanins in blue and purple wheat grains were clarified. A rapid method for the separation and identification of anthocyanins in blue and purple wheat grains by mass spectrometry was established.