论文部分内容阅读
目的:通过通脉地仙丸作用于心肌缺血的大鼠,观察缺血心肌梗死边缘区的微血管新生情况,初步探讨其心肌保护机制。方法:100只雄性大鼠慢性心肌缺血造模成功后除假手术组外随机分为模型组,通脉地仙高、中、低(6.3,3.15,1.57 g·kg-1)剂量组,阳性药麝香保心丸(0.014 2 g·kg-1)组。灌胃给药6周后观察大鼠对心肌梗死范围的影响,同时苏木精-伊红(HE)染色观察心肌组织病理形态学的改变,并免疫组织化学检测观察心肌梗死边缘区微血管新生情况。结果:①与模型组比较,通脉地仙高、中剂量组和麝香保心丸组心肌梗死面积不同程度减小(P<0.05,P<0.01)。②通脉地仙丸对大鼠心肌组织的影响:光镜下观察,假手术组心肌纤维排列整齐且无肿胀现象,心肌间质极少炎性细胞浸润;模型组心肌纤维肿胀,排列紊乱,心肌间质大量炎细胞浸润,细胞核大小不一,甚至可见局灶性坏死区;通脉地仙高、中剂量组心肌细胞核大小均一,心肌纤维轻度肿胀,排列比较整齐,仍可见不同程度的成纤维细胞及炎细胞浸润;阳性对照组镜下心肌纤维排列基本整齐,轻度肿胀,可见少量炎细胞浸润。③缺血心肌血管新生的微血管密度(MVD)比较:与假手术组比较,模型组大鼠微血管密度相对增加(P<0.05),通脉地仙高、中剂量组和阳性对照组与模型组比较有明显微血管密度增加的趋势(P<0.01),通脉地仙低剂量组MVD也有不同程度的增加微血管的作用,但与模型组比较没有统计学差异。结论:通脉地仙丸可以通过促进心梗边缘区微血管的生成从而达到保护心肌组织和细胞的目的。
OBJECTIVE: To observe the angiogenesis in ischemic myocardium in rats with myocardial ischemia by means of Tongmai Xianxian Pill, and to explore the mechanism of myocardial protection. Methods: One hundred male rats were randomly divided into model group, high dose, middle dose and low dose (6.3,3.15,1.57 g · kg-1) Positive drug musk heart pill (0.014 2 g · kg-1) group. Six weeks after intragastric administration, the myocardial infarct size was observed. The changes of myocardial histomorphology were observed by hematoxylin-eosin (HE) staining and the angiogenesis was observed by immunohistochemistry . Results: ① Compared with the model group, the area of myocardial infarction in Tongmai Decoction high dose group, middle dose group and Shexiang Baoxin Pill group decreased to some extent (P <0.05, P <0.01). ②The effect of Tongmaidixian Pills on myocardial tissue in rats: Under the light microscope, the myocardial fibers in the sham-operated group were arranged neatly with no swelling, infiltration of inflammatory cells with minimal myocardial interstitium; the myocardial fibers in the model group were swollen and disordered, Myocardial interstitium infiltration of a large number of inflammatory cells, the nuclei vary in size, and even visible focal necrosis; Tongmaidi high, medium dose myocardial cell nucleus size uniformity, myocardial fiber mild swelling, arranged more neat, still visible to varying degrees Fibroblasts and inflammatory cells infiltration; positive control group myocardial fibers arranged in regular neat, mild swelling, showing a small amount of inflammatory cell infiltration. Compared with the sham-operation group, the microvessel density in the model group was increased (P <0.05), and that in the high-dose, middle-dose and positive control groups was significantly higher than that in the model group Compared with the model group, the MVD in the low-dose Tongmaidian low-dose group showed a significant increase in microvessel density (P <0.01), but there was no significant difference compared with the model group. Conclusion: Tong Mai Di Xian Wan can promote myocardial tissue and cells by promoting the formation of microvascular in marginal zone of myocardial infarction.