目的　建立检测HFRS患者血清标本中HV基因的PCR -ELISA方法。方法　设计并合成互补于HVS基因片段的标记引物 ,对 98例HFRS患者血清进行RT -nestedPCR扩增 ,并用酶免方法分析扩增产物。结果　 98例患者不同病日采集的血清标本的RT -nestedPCR平均检出率仅为 6 0 .2 % ,而PCR -ELISA平均检出率为 80 .6 %。结论　PCR -ELISA用于早期HFRS患者的HV基因检测是一种理想的检测方法 Objective To establish a PCR-ELISA method to detect HV gene in serum of HFRS patients. Methods Primer primers complementary to HVS gene fragments were designed and synthesized. The serum of 98 patients with HFRS was amplified by RT-nested PCR, and the amplification products were analyzed by enzyme-free method. Results The average detection rate of RT -nestedPCR in sera of 98 patients collected on different days was only 62.2%, while the average detection rate of PCR-ELISA was 80.6%. Conclusion PCR-ELISA for detecting HV genotypes in patients with early HFRS is an ideal test