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目的研究通络方药(TLR)对链脲佐菌素(STZ)所致大鼠胰岛β细胞损伤的保护作用。方法建立糖尿病大鼠模型前8天开始给予TLR直至实验结束。实验结束时测血糖、体重;取胰腺测定胰腺匀浆中丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)水平,透射电镜检测胰岛β细胞。结果与正常组相比,STZ组胰腺内MDA水平明显升高(P<0.05),GSH-Px活性明显降低(P<0.05),血糖明显升高。TLR预处理明显降低STZ组大鼠的血糖水平,同时降低大鼠胰腺匀浆内MDA含量(P<0.05),升高GSH-Px活性(P<0.05)。透射电镜显示TLR预处理使胰岛β细胞的损伤明显减轻。结论 TLR通过减轻胰腺内氧化应激水平保护胰岛β细胞完整并有效防止STZ诱导的糖尿病发生。
Objective To study the protective effect of Tongluo prescription (TLR) on islet β-cell injury induced by streptozotocin (STZ) in rats. Methods The TLR was started 8 days before the establishment of the diabetic rat model until the end of the experiment. At the end of the experiment, blood glucose and body weight were measured; pancreas was taken to determine the level of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in the pancreas homogenate, and islet β cells were detected by transmission electron microscope. Results Compared with the normal group, the levels of MDA in the pancreas of STZ group were significantly increased (P<0.05), GSH-Px activity was significantly decreased (P<0.05), and blood glucose was significantly increased. TLR pretreatment significantly reduced the blood glucose level in rats of STZ group, and reduced the content of MDA in the pancreas homogenate (P<0.05) and increased the activity of GSH-Px (P<0.05). Transmission electron microscopy showed that pretreatment with TLR significantly reduced islet beta cell injury. Conclusion TLR can protect pancreatic islet β-cell integrity by reducing oxidative stress in pancreas and effectively prevent STZ-induced diabetes.