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目的 探讨CODIS系统中的9个STR基因座及Amel基因座在肿瘤组织中的变异。方法 收集20个个体的肿瘤组织及其血样,Chelex100法提取DNA,用Profiler plus系统复合扩增,310型遗传分析仪检测。结果 检验的10个基因座均有基因发生突变;20个个体肿瘤组织有6个个体的基因发生突变,变异率超过30％;同一个体的肿瘤组织发生基因突变的基因座最多为6个。结论 慎用肿瘤组织作为医疗纠纷、失踪人员的检材及对照样本。
Objective To investigate the variation of 9 STR loci and Amel loci in tumor tissues in CODIS system. Methods The tumor tissues and blood samples of 20 individuals were collected. The DNA was extracted by Chelex100 method and amplified by Profiler plus system. The results of the test of the 10 loci have gene mutations; 20 individual tumor tissue 6 individuals have mutations in the gene mutation rate of more than 30%; the same individual tumor gene mutation loci up to 6. Conclusion Careful use of tumor tissue as a medical dispute, missing staff samples and control samples.