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为进一步研究苹果斑点落叶病感病性遗传规律,采用1株苹果斑点落叶病菌株对‘紫塞明珠’ב富士’(Malus asiatica×M.domestica)的1 071株杂交实生树的离体叶片进行斑点落叶病的接种鉴定,将表型数据结合本实验室之前构建的遗传连锁图谱及基因型数据,进行QTL区间作图分析。采用Regression和Mixture model 2种算法共得到与苹果斑点落叶病感病性相关的QTL位点18个,分布在LG07、LG08、LG09、LG12和LG17等5个连锁群上。Mixture model算法与Regression算法所得微效QTL位点重叠或完全重合。Regression算法未能定位到主效QTL位点,而Mixture model算法在发病率和严重度2个感病指标均检测到2个主效QTL位点。发病率与病情指数性状的遗传控制基本相同,严重度则表现为相对独立性状。
In order to further study the genetic inheritance of susceptibility to apple leaf spotted leaf decay, 1 leaf spot of apple blight was used to isolate 1071 hybrid seedling leaves of ’Malus asiatica × M.domestica’ Inoculation of spotted leaf deciduous disease was carried out. The phenotypic data were combined with the genetic linkage map and genotype data constructed in our laboratory before QTL interval mapping. A total of 18 QTLs related to the susceptibility to apple leaf spot disease were obtained using Regression and Mixture model, which were distributed on 5 linkage groups LG07, LG08, LG09, LG12 and LG17. Mixture model algorithm and Regression algorithm derived from micro-QTL sites overlap or completely coincide. Regression algorithm failed to locate the major QTLs, while the Mixture model detected two major QTLs in both susceptibility and severity. The incidence of disease and disease index trait genetic control is basically the same, the severity of the performance of relatively independent traits.