论文部分内容阅读
目的探讨经亚胺培南(IMP)诱导产生耐药的铜绿假单胞菌(PA)的耐药表型及其耐药机制,旨在为临床有效治疗PA感染提供理论依据。方法收集2014年8月-2016年8月从临床送检标本中分离的抗菌药物全敏的PA株共计64株,以最终抗菌药物全敏的PA 62株临床分离株为实验菌株,分析比较各耐药相关表型在其中的作用,采用WHONET软件对数据进行统计分析。结果经人工诱导25代后获得的菌株都具有IMP耐药性;产MBL的IMP耐药PA(IRPA)47株,占75.8%;产ESBLs的IRPA 21株,占33.8%;产AmpC的IRPA 9株,占14.5%;主动外排系统表型阳性IRPA 3株,占4.8%。结论 PA产生IMP耐药性涉及MBL、ESBL、AmpC和主动外排系统激活等多种耐药机制,本组诱导耐药表型以MBL阳性为主。
Objective To investigate the drug resistance phenotype and its drug resistance mechanism of drug-resistant Pseudomonas aeruginosa (PA) induced by imipenem (IMP), and to provide a theoretical basis for clinical effective treatment of PA infection. Methods A total of 64 antimicrobial PA strains isolated from clinical samples were collected from August 2014 to August 2016. The PA62 clinical isolates with full antimicrobial susceptibility were used as experimental strains, The role of resistance-associated phenotypes in the data was analyzed using WHONET software. RESULTS: All strains obtained after artificial induction for 25 generations had IMP resistance; 47 strains of IMP resistant MB with MBL accounted for 75.8%; 21 strains of IRPA producing ESBLs accounted for 33.8%; AmpC-producing IRPA 9 Strain, accounting for 14.5%; active efflux system phenotype positive IRPA 3, accounting for 4.8%. Conclusion The PA-resistance in PA involves multiple drug resistance mechanisms such as MBL, ESBL, AmpC and active efflux system activation. The dominant drug-resistant phenotype in this group is MBL positive.