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为分析目前广泛使用的3型脊髓灰质炎病毒减毒活疫苗(OPV)株的基因突变趋势,作者使用了一种灵敏的检测方法:即利用聚合酶链反应(PCR)和限制性内切酶裂解分析突变(MAPREC)的方法。分析对象为多个批号的商品Sabin3型活疫苗,分别来自原代病毒株(SO)和RNA蚀斑纯化的病毒株(RSO)。细胞基质分别是原代非洲绿猴肾(AGMK)细胞和Vero细胞。当这些毒株在细胞中传10代后,利用上述方法可检出14个新的突变,其中9个可在一种以上培养条件下出现,另5个只在一种培养条件下出现。3956核苷酸位点上的A→G、5788位点上的T→C和6979位点上的T→C突变仅见于
In order to analyze the gene mutation trend of the attenuated live vaccine (OPV) strain of poliovirus type 3 currently widely used, the authors used a sensitive detection method that uses polymerase chain reaction (PCR) and restriction endonuclease Method of lysis analysis of mutations (MAPREC). The analysis was performed on multiple lots of commercial Sabin3 live vaccines derived from primary virus (SO) and RNA plaque purified virus (RSO), respectively. The cell matrices were primary African green monkey kidney (AGMK) cells and Vero cells, respectively. When these strains were passaged for 10 passages in the cell, 14 new mutations were detected using the above method, of which 9 appeared in more than one culture and the other only in one culture. A → G at 3956 nucleotide positions, T → C at 5788 and T → C mutations at 6979 were only found in