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本试验采用SDS－PAGE对10株嗜水气单胞菌（Ah）的外膜蛋白（OMP）和脂多糖（LPS）进行分析，结果显示大多数菌株具有相似的OMP图谱，而LPS则有较大的差异，用鲫鱼抗AhJ-1株全菌血清进行免疫转印，发现S蛋白（S－layerProtein）是70％Ah菌株的共同抗原，而LPS则有明显的抗原多样性，AhJ－1株抗血清仅能与同源菌株起反应。用AhJ－1株HEC毒素（Hemolvticactivity，Enterotoxicity，Cytotoxicity，HEC）的单克隆抗体检测此10株Ah，8株细菌可产生抗原性相同的HEC毒素。用纯化的AhJ－1株的S蛋白、LPS及HEC毒素（预先天活）分别免疫小鼠，强化免疫后，对同源菌株的攻击均能完全保护；S蛋白免疫组和HEC毒素免疫组对异源菌株TPS－30的攻击均能保护，而LPS免疫组则不能完全保护。上述结果表明，S蛋白和HEC毒素是Ah的主要共同保护抗原。AhJ-1株有望成为预防嗜水气单胞菌败血症的疫苗生产菌株。
In this study, OMP and LPS of 10 strains of A. hydrophila were analyzed by SDS-PAGE. Most of the strains showed similar OMP profiles, while LPS had more The results showed that S-layer protein was the common antigen of 70% Ah strain, while LPS had obvious antigenic diversity. AhJ-1 strain Antisera can only react with homologous strains. The McAbs of AhJ-1 HEC toxin (Hemolvtic activity, Enterotoxicity, Cytotoxicity, HEC) detection of 10 strains of Ah, 8 strains of bacteria can produce HEC toxins with the same antigenicity. Immunization of mice with purified protein S, LPS and HEC toxin (prenatal live) of AhJ-1 strain, respectively, gave complete protection against challenge with the homologous strain after challenge with immunization; S protein immunization group and HEC toxin immunization group The heterologous strain TPS-30 attack was able to protect, while the LPS immune group was not completely protected. The above results indicate that S protein and HEC toxin are the major co-protective antigens of Ah. The strain AhJ-1 is expected to become a vaccine production strain for preventing Aeromonas hydrophila septicemia.