【摘 要】
：
OBJECTIVE URB597(KDS-4103)is a potent and selective inhibitor of the enzyme fatty acid amide hydrolase(FAAH)and can ele⁃vate the level of oleoylethanolamide(OEA),a naturally occurring endocannabinoid in the brain.However,the effect of URB597 on cerebral i
【机 构】
：
DepartmentofBasicMedicalScience,KeyLaboratoryofChiralDrugs,StateKeyLaboratoryofCellularStressBiology
【基金项目】
：
National Natural Science Foundation of China(81603093),and the Open Research Fund of State Key Laboratory of Cellu⁃lar Stress Biology,Xiamen University(SKLC⁃SB2019KF016)。
论文部分内容阅读
OBJECTIVE URB597(KDS-4103)is a potent and selective inhibitor of the enzyme fatty acid amide hydrolase(FAAH)and can ele⁃vate the level of oleoylethanolamide(OEA),a naturally occurring endocannabinoid in the brain.However,the effect of URB597 on cerebral i
其他文献
目的当今,更多人群因常常需要在狭小环境中久坐工作,长期作息不规律且缺乏活动而导致疲劳。本研究希望通过慢性束缚应激(CRS)模拟以上人群疲劳的发生情况,并对CRS小鼠疲劳模型的动态发生及机制进行探索。方法将48只4周龄雄性ICR小鼠按体重和前肢抓力随机分成4组,分别束缚0,5,10和15 d,8 h·d-1。束缚结束后进行空场实验、抓力实验和负重游泳实验。次日各组小鼠强迫游泳30 min后休息30 min,戊巴比妥钠麻醉后从腹主动脉取血,脱颈处死取肝和后肢腓肠肌组织用于生化指标检测。结果与空白组相比,CRS
目的CRISPR介导的基因编辑对基因治疗带来了巨大的前景,目前已有多项基于CRISPR的研究用于阿尔茨海默症的治疗。然而目前将CRISPR递送到相应的细胞甚至体内应用依然缺乏安全有效的手段,在细胞层面目前主要依赖于电转手段将CRISPR系统运送到细胞中,操作上比较复杂。而基于慢病毒和AAV的CRISPR递送体系不仅会造成免疫原性,而且往往容易整合到基因组中长期表达,引发不良后果。本研究拟基于外泌体的递送策略改善该问题。方法本论文采用构建工程化的外泌体平台,采用基于com和配体结合蛋白Com的富集系统,可以
目的脑卒中是全球死亡和残疾的主要原因,约占全球5%的伤残调整年和10%的死亡率。目前临床上脑卒中的治疗存在有效治疗手段的局限性,除溶栓外仍缺乏有效的神经保护及再生修复治疗手段,且治疗时间窗窄,临床预后改善率低。脑卒中后存活患者不同程度丧失劳动力,其中上肢运动功能障碍在首次脑卒中后生存的患者中占73%~88%,在慢性脑卒中中占到55%~75%。寻找有效的神经再生修复治疗新方法,打破脑卒中治疗的瓶颈,是脑卒中后康复治疗的重点目标,对于最大程度促进患者功能恢复、减轻脑卒中负担有极为重要的意义。脑深部电刺激(DB
OBJECTIVE Ginsenoside Rb1(Rb1),an important bioactive ingredient of Panax ginseng,has potent neuroprotective effects.The objective of the study is to elucidate the impact of Rb1 treatment on chronic social defeat stress(CSDS)-induced depressive-like behav
OBJECTIVE To clarify the role of translocator protein 18 ku(TSPO)on cecum liga⁃tion and puncture(CLP)induced sepsis associat⁃ed encephalopathy(SAE)mice,which consis⁃tently demonstrated astrocyte activation and neu⁃roinflammation.Background SAE,a brain dys
OBJECTIVE A novel mast cell-specific G-protein-coupled receptor(GPCR),known as mas-related GPCR-B2(MRGPRB2),plays important roles in the immune response.The opening of ion channels mediated by MRGPRB2 activation remains unclear.METHODS AND RE⁃SULTS Calciu
目的探索中脑导水管周围灰质腹外侧部(vlPAG)星形胶质细胞活化在糖尿病神经病理性疼痛(DNP)及痛相关负性情绪发生、发展及维持过程中的具体作用及特征。方法使用链脲佐菌素(STZ)诱导SD大鼠Ⅰ型糖尿病,复制DNP模型;免疫荧光法观察、Sholl分析评价vlPAG星形胶质细胞在DNP进展过程中的形态和数量变化;采用化学遗传学技术定点定量向vlPAG注射携有星形胶质细胞特异性启动子gfaABC1D及修饰后的人毒蕈碱受体(hM3Dq或hM4Di)基因片段的腺相关病毒;待病毒稳定转染表达后,利用受体的外源性配体
OBJECTIVE Previous studies have demonstrated a close association between an altered immune system and major depressive disorders,and inhibition of neuroinflammation may represent an alternative mechanism to treat depression.Recently,the anti-inflammatory
OBJECTIVE Genetic variation in histamine H2 receptor(H2R)and H2R ligands are linked to schizophrenia,however little is known about how H2R contributes to pathogenesis of schizophrenia.Here,we detected a decreased expression of H2R in medial prefrontal cor
OBJECTIVE To identify benzodi⁃azepine(BZD)effects that are insensitive to the classical BZD binding site antagonist,flumazenil.Whether the flumazenil-insensitive BZD effects have selectivity on different GABAA receptor sub⁃types was also investigated.METH