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目的从新疆光果甘草中制备总黄酮(LGFs)及单体成分光甘草定(glabridin,GLB),并研究它们的抗氧化活性。方法用常规方法制备LGFs和GLB;利用HL-60细胞中的Myeloperoxidase/H2O2/HClO氧化系统和肝微粒体中的细胞色素P450/NADPH氧化系统做体外抗氧化实验模型,对LGFs和GLB的抗氧化活性进行测定。系统中自由基诱发程度由探针物DCFH-DA的氧化产物DCF的浓度来检测。银杏叶提取物EGb761用作阳性对照物。结果在两种体外模型中,LGFs和GLB均显示较强的抗氧化活性,它们清除自由基活性强度为EGb761≥GLB>LFs。结论甘草总黄酮及其中的主要活性成分GLB在HL-60细胞和肝微粒体氧化系统中具有很强的抗氧化活性。这些结果可部分解释甘草药理作用与其抗氧化预防活性的关系。
Objective To prepare total flavonoids (LGFs) and monomeric constituents glabricin (GLB) from Glycyrrhiza glabra in Xinjiang and study their antioxidant activities. Methods LGFs and GLB were prepared by conventional methods; In vitro antioxidative assay model was performed using the Myeloperoxidase/H2O2/HClO oxidation system in HL-60 cells and the cytochrome P450/NADPH oxidation system in liver microsomes to antioxidation of LGFs and GLB. Activity was measured. The extent of free radical induction in the system is measured by the concentration of DCF, the oxidation product of the probe DCFH-DA. Ginkgo biloba extract EGb761 was used as a positive control. Results In both in vitro models, LGFs and GLB showed strong antioxidant activity. Their scavenging free radical activity was EGb761≥GLB>LFs. Conclusion Glycyrrhiza uralensis Flavone and its main active ingredient GLB have strong antioxidant activity in HL-60 cells and liver microsome oxidation system. These results can partly explain the relationship between the pharmacological effects of licorice and its antioxidant activity.