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近年来,随着分子生物学的飞速进步,在寄生虫学领域也已应用基因工程技术来分析寄生现象。当前还尝试开发新的诊断技术,其中之一就是采用DNA探针作寄生虫检查。用这一方法研究较多的寄生虫病是疟疾,其次是利什曼病和盘尾丝虫病。此外,这种方法还有其它广泛应用,如在马来丝虫的媒介昆虫蚊体内,要将感染幼虫与其它形态学上唯以区别的近缘丝虫的幼虫作鉴别,就可用该方法来作鉴定。本文主要讨论DNA探针检查法在疟疾、利什曼病和盘尾丝虫病诊断中的应用,就其原理和意义作一说明。
In recent years, with the rapid advances in molecular biology, genetic engineering has also been applied to analyze parasitism in the field of parasitology. One of the current attempts to develop new diagnostic techniques is the use of DNA probes for parasite testing. More parasites were malaria in this method, followed by leishmaniasis and onchocerciasis. In addition, this method has many other applications. For example, in the case of a vector insect mosquito of a malayian worm, this method can be used to discriminate infected larvae from larvae of other morphologically distinct closely related filarial worms For identification. This article mainly discusses the application of DNA probe test in the diagnosis of malaria, leishmaniasis and onchocerciasis, and explains its principle and significance.