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目的:探讨苦参碱(matrine,Mat)对人淋巴瘤细胞株(U937)的抗癌作用及其分子机制。方法:用0.1,0.2,0.3,0.4,0.5 g.L-1Mat处理U937细胞后,分别采用倒置显微镜和电子显微镜观察细胞形态学变化;台盼蓝拒染法及MTT实验检测细胞增殖抑制作用;Western blot方法检测细胞MAPK的磷酸化活性。结果:0.2 g.L-1Mat对人组织淋巴瘤U937细胞的增殖有抑制作用;0.2 g.L-1Mat作用U937细胞48 h后,倒置显微镜下可见细胞数目减少,有些细胞变小、变圆,随着Mat作用浓度的增加,U937细胞逐步出现增多的凋亡细胞;Mat可以抑制U937细胞中ERK的活性,并在一定程度上提高p38和JNK的活性。结论:Mat可以在体外抑制人淋巴瘤细胞U937的增殖作用,诱导其凋亡,并且Mat可能通过抑制U937细胞中ERK的活性,提高p38和JNK的活性发挥其诱导凋亡作用。
Objective: To investigate the antitumor effect of matrine (Mat) on human lymphoma cell line (U937) and its molecular mechanism. Methods: U937 cells were treated with 0.1, 0.2, 0.3, 0.4 and 0.5 gL-1 Mat respectively. Morphological changes were observed by inverted microscope and electron microscope. Cell proliferation was detected by trypan blue exclusion assay and MTT assay. Western blot Methods The phosphorylation activity of MAPK in cells was detected. Results: 0.2 gL-1Mat could inhibit the proliferation of U937 cells. After U937 cells were treated with 0.2 gL-1Mat for 48 h, the number of cells decreased and some cells became smaller and round with inverted microscope. U937 cells gradually increased the number of apoptotic cells; Mat inhibited the activity of ERK in U937 cells and increased the activity of p38 and JNK to a certain extent. Conclusion: Mat can inhibit the proliferation of human lymphoma U937 cells in vitro and induce apoptosis. Mat may inhibit the activity of ERK in U937 cells and increase the activity of p38 and JNK to induce apoptosis.