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建立了罗非鱼(Oreochromis niloticus×O.aureus)血浆、肌肉和肝脏内氟甲砜霉素的高效液相色谱测定方法(HPLC法)。采用内标法和峰高法定量,方法准确,重复性好,灵敏度较高。8、1和0.125μg·mL-13个浓度水平的加标回收率为89.16%-96.13%;对应的批内精密度和批间精密度分别为2.21%-6.55%和2.11%- 7.71%。方法检出限为0.03μg·mL-1。结果表明,此方法非常适合用于罗非鱼体内氟甲砜霉素的动力学和残留研究。
A high performance liquid chromatography (HPLC) method for the determination of florfenicol in plasma, muscle and liver of Oreochromis niloticus × O.aureus was established. Using internal standard method and peak height method, the method is accurate, good repeatability and high sensitivity. The spiked recoveries of 8,1 and 0.125μg · mL-13 were 89.16% -96.13%. The corresponding intra-assay precision and inter-assay precision were 2.21% -6.55% and 2.11% -7.71% respectively. The detection limit was 0.03μg · mL-1. The results show that this method is very suitable for the kinetics and residue studies of florfenicol in tilapia.