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目的:探讨三种低氧训练模式对大鼠腓肠肌有氧代谢酶活性的影响。方法:经过适应性训练和力竭实验筛选出40只雄性SD大鼠,采用双盲法平均分成4组:低住低练组、高住高练组、高住低练组和低住高练组。采用水平动物跑台进行耐力训练,运动强度为常氧下35m/min、低氧下30m/min,1h/d,5d/周,持续训练6周。低住低练组大鼠在常氧环境下生活训练;高住高练组大鼠在低氧环境(氧浓度为13.6%,约相当于海拔3500m高度)生活训练;高住低练组大鼠低氧环境生活12h,常氧环境训练;低住高练组大鼠在常氧环境生活,低氧环境训练。最后一次训练后恢复48h取腓肠肌,检测柠檬酸合成酶(CS)、琥珀酸脱氢酶(SDH)和苹果酸脱氢酶(MDH)活性。结果:与低住低练组比较,高住高练组大鼠腓肠肌CS、SDH和MDH活性分别升高11.7%(P<0.01)、8.7%(P<0.05)和12.5%(P<0.01);高住低练组、低住高练组较低住低练组增加,但无统计学意义。结论:3500米三种低氧训练模式就提高机体有氧代谢酶活性而言,高住高练优于高住低练和低住高练。
Objective: To investigate the effects of three hypoxic training modes on the aerobic metabolism enzyme activity of gastrocnemius muscle in rats. Methods: Forty male SD rats were screened by adaptive training and exhaustive test. The rats were equally divided into four groups by double-blind method: low-abortion training group, high-living training group, high-living training group and low abortion training group group. Endurance training was carried out with horizontal animal treadmill. The exercise intensity was 35m / min under normoxia, 30m / min under hypoxia, 1h / d, 5d / week, and continued training for 6 weeks. Living low-training low-training group of rats living training in normoxia; Living high-training group of rats in hypoxia environment (oxygen concentration of 13.6%, equivalent to an altitude of 3500m height) Hypoxic environment 12h, training in normoxic environment; living in high training group living in normoxic environment, hypoxic environment training. The gastrocnemius was recovered 48h after the last training, and the activities of citrate synthase (CS), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) were detected. Results: The CS, SDH and MDH activity of gastrocnemius muscle of Guoxiazhanglian training group increased by 11.7% (P <0.01), 8.7% (P <0.05) and 12.5% (P <0.01) ; High living low training group, living low training low training group to increase, but no statistically significant. Conclusion: The three hypoxia training modes of 3,500 meters increase aerobic metabolism enzyme activity in the body.