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可溶性肿瘤坏死因子相关诱导配体(sTRAIL)因对多种肿瘤细胞具有超强选择性杀伤活性而极可能成为新型抗肿瘤药物。基因工程重组表达sTRAIL过程中缺乏锌离子(Zn2+)可能是导致该蛋白对肿瘤细胞杀伤活性较低的重要原因。本文通过化学合成方法获得了sTRAIL编码基因,将其克隆到pQE30载体中获得了高表达。比较不同Zn2+浓度条件下制备的sTRAIL蛋白分子聚合状态和肿瘤细胞杀伤活性发现,无Zn2+的sTRAIL主要以单体形式存在,活性弱;有Zn2+的sTRAIL主要以同源三聚体形式存在,活性强。这表明Zn2+对维持sTRAIL肿瘤细胞杀伤活性极为重要,有必要在sTRAIL蛋白生产体系中添加适当浓度的Zn2+。
Soluble tumor necrosis factor-related inducible ligand (sTRAIL) is highly promising as a novel antitumor drug due to its super selective activity against a variety of tumor cells. The lack of zinc ion (Zn2 +) in genetically engineered recombinant sTRAIL may be an important reason for the low cytotoxic activity of this protein on tumor cells. In this paper, sTRAIL encoding gene was obtained by chemical synthesis and cloned into pQE30 vector to get high expression. The results showed that sTRAIL without Zn2 + mainly existed in monomer form and had weak activity. STRAIL with Zn2 + mainly existed in the form of homotrimer with strong activity . This indicates that Zn2 + is crucial for maintaining the cytotoxic activity of sTRAIL tumor cells and it is necessary to add appropriate concentrations of Zn2 + to the sTRAIL protein production system.