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采用高效液相色谱仪 (HPLC ,UV检测器 )测定面粉及其制品中的过氧化苯甲酰 (BPO)含量。用C18色谱柱 ,以甲醇 - 0 .0 2mol/L乙酸铵 (5 +95 )为流动相 ,检测波长 2 30nm ,以保留时间作定性 ,以峰面积或峰高作定量 ,以苯甲酸为标准 ,计算样品中BPO含量。结果显示在 0～ 10 0mg/L范围内呈良好线性关系 (r=0 .9995 ) ;检测限为 0 .2mg/L ,方法的回收率为 93.5 %～ 110 .8% ;相对标准偏差平均为 2 .6 %。结果表明 ,本法简单、快速、准确 ,易于操作 ,用于面粉及其制品BPO的测定 ,结果满意
High performance liquid chromatography (HPLC, UV detector) was used to determine the content of benzoyl peroxide (BPO) in flour and its products. With C18 column, methanol - 0.02mol / L ammonium acetate (5 +95) as the mobile phase, the detection wavelength of 230nm, with retention time as a qualitative, peak area or peak height as the standard, with benzoic acid as a standard , Calculate the BPO content in the sample. The results showed that there was a good linear relationship (r = 0.9995) in the range of 0 ~ 100mg / L; the detection limit was 0.2mg / L, the recovery rate was 93.5% ~ 110.8%; the average relative standard deviation was 2 .6%. The results show that this method is simple, rapid, accurate and easy to operate and is used for the determination of BPO in flour and its products. The results are satisfactory