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目的:探讨脓毒血症患者血小板骨架蛋白Gelsolin和造血系细胞特异性蛋白(HS-1)及其和凝血功能的关系。方法:纳入脓毒血症患者30例和对照组健康人群30例,用双抗体夹心法测定血浆中血小板骨架蛋白,用酶联免疫法测定血清中HS-1蛋白含量,采用免疫荧光观察血小板Gelsolin蛋白的表达,分析Gelsolin、HS-1、血小板计数和凝血功能(PT、APTT)之间的关系。结果:对照组Gelsolin主要位于血小板胞浆内,观察组包浆内外及间质内均可见。观察组血小板骨架蛋白Gelsolin、HS-1水平明显高于对照组,差异均存在统计学意义(P<0.05)。观察组血小板计数、PT、APTT水平均低于对照组,差异存在统计学意义(P<0.05)。Galsolin和血小板计数呈负相关关系,相关系数r=-0.76(P<0.05),HS-1和血小板计数间呈负相关关系相关系数r=-0.69(P<0.05)。结论:早期脓毒血症患者血小板骨架蛋白Gelsolin和HS-1表达水平明显升高,与脓毒血症患者血小板大量破坏有关。
Objective: To investigate the relationship between platelet-derived protein Gelsolin and hematopoietic cell-specific protein (HS-1) in sepsis and its relationship with coagulation function. Methods: Thirty patients with sepsis and 30 healthy subjects were enrolled in this study. Plasma platelet-derived protein was detected by sandwich enzyme-linked immunosorbent assay (ELISA). Serum levels of HS-1 protein were measured by enzyme-linked immunosorbent assay. Gelsolin Protein expression, analyze the relationship between Gelsolin, HS-1, platelet count and coagulation function (PT, APTT). Results: Gelsolin in the control group was mainly located in the cytoplasm of platelets, both in the cytoplasm and in the interstitium in the observation group. The levels of Gelsolin and HS-1 in the observation group were significantly higher than those in the control group, with statistical significance (P <0.05). The platelet count, PT and APTT levels in the observation group were lower than those in the control group, with statistical significance (P <0.05). Galsolin had a negative correlation with platelet count (r = -0.76, P <0.05). Correlation coefficient between HS-1 and platelet counts was -0.69 (P <0.05). Conclusion: The expression of Gelsolin and HS-1 in patients with early sepsis is significantly increased, which is related to the massive destruction of platelets in patients with sepsis.