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①目的探讨ＶＰ１６对细胞凋亡的作用。②方法用两种剂量（３．１２，６．２５μｍｏｌ）的ＶＰ１６处理腹腔接种Ｓ－１８０瘤细胞和抗阿霉素瘤细胞（Ｓ－１８０－Ｒ）的ＢＡＢＬ／Ｃ鼠。０，６，９ｈ分别取腹水，用流式细胞仪测定细胞相对ＤＮＡ含量。③结果ＶＰ１６在３．１２，６．２５μｍｏｌ剂量时，均可诱导Ｓ－１８０细胞凋亡，而剂量效应并不明显。在６．２５μｍｏｌ时，也可诱导Ｓ－１８０－Ｒ细胞凋亡，且ＶＰ１６诱导Ｓ－１８０和Ｓ－１８０－Ｒ两种细胞凋亡差别不明显。④结论ＶＰ１６是Ｓ－１８０和Ｓ－１８０－Ｒ细胞凋亡的诱导剂。ＶＰ１６诱导的凋亡与其抗药表型关系不大，但与免疫机制似乎有一定的关系。
Objective To investigate the effect of VP16 on apoptosis. 2 Methods S-180 tumor cells and BABL/C mice resistant to doxorubicin tumor cells (S-180-R) were intraperitoneally inoculated with two doses (3.12, 6.25 μmol) of VP16. Ascites fluid was taken at 0, 6, and 9h respectively, and the relative cell DNA content was measured by flow cytometry. 3 Results At doses of 3.12 and 6.25μmol, VP16 could induce apoptosis of S-180 cells, but the dose effect was not obvious. At 6.25 μmol, apoptosis of S-180-R cells was also induced, and there was no significant difference between VP16-induced apoptosis of S-180 and S-180-R cells. 4 Conclusions VP16 is an inducer of apoptosis in S-180 and S-180-R cells. The apoptosis induced by VP16 was not related to its resistance to phenotype, but it seems to have a certain relationship with the immune mechanism.