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背景:在脑血管病脑损伤中有许多细胞因子的参与,而白细胞介素6(interleukin-6,IL-6)被认为在中枢神经系统中具有神经保护和神经营养作用,而脑出血急性期脑脊液可溶性IL-6受体(solubleinterleukin-6re-ceptor,sIL-6R)的研究国内外报道较少。目的:研究脑出血急性期脑脊液中sIL-6R变化与继发性脑损伤的关系。设计:病例对照研究。单位:暨南大学医学院第五附属医院检验科、神经外科、神经内科联合进行课题研究。对象:从1999-01/2003-12收治的脑出血患者627例中,选择经临床诊断和CT证实的自发性脑出血后48h内入院的患者,并排除创伤性脑出血和其他脑血管疾病,符合以上标准的32例患者作为研究对象(脑出血组),其中脑实质出血破入脑室者23例,单纯脑实质出血者7例,单纯脑室出血2例。13例同期住院的非神经系统疾病就诊者为对照组。方法:患者于入院后2d内腰穿取脑脊液2mL离心沉淀取上清液放-80℃超低温冰箱保存待测,12例患者出血后第1,2,3,5,10～13天进行动态观察。采用酶联免疫吸附试验(ELISA)测定对照组和脑出血患者脑脊液中sIL-6R含量。主要观察指标:对照组和脑出血患者脑脊液中sIL-6R含量。结果:13例对照组和32例脑出血急性期患者脑脊液中sIL-6R含量分别为(380.54±93.05)ng/L,(1220.18±878.71)ng/L,两者之间差?
BACKGROUND: There are many cytokines involved in cerebrovascular disease and brain injury, whereas interleukin-6 (IL-6) is thought to have neuroprotective and neurotrophic effects in the central nervous system. However, acute cerebral hemorrhage CSF IL-6 receptor (solubleinterleukin-6re-ceptor, sIL-6R) research reports at home and abroad less. Objective: To study the relationship between changes of sIL-6R in cerebrospinal fluid and secondary brain injury in patients with acute cerebral hemorrhage. Design: Case-control study. Unit: Affiliated Hospital of Jinan University, the Fifth Affiliated Hospital of Neurology, Neurology, joint research project. PARTICIPANTS: A total of 627 ICH patients admitted from January 1999 to December 2003 were enrolled in this study within 48 hours after spontaneous intracerebral hemorrhage confirmed by clinical diagnosis and CT, and traumatic cerebral hemorrhage and other cerebrovascular diseases were excluded. Thirty-two patients who met the above criteria were selected as study subjects (intracerebral hemorrhage group). Among them, 23 patients had intracerebral hemorrhage of brain parenchyma, 7 patients had simple parenchymal hemorrhage and 2 patients had simple intraventricular hemorrhage. Thirteen patients hospitalized with non-neurological diseases in the same period were selected as the control group. Methods: 2d after admission, the patients were subjected to lumbar puncture and cerebrospinal fluid 2mL centrifuged to collect the supernatant and stored at -80 ℃ in a cryogenic refrigerator for testing. Twelve patients were dynamically observed on the 1st, 2nd, 3rd, 5th and 10th to 13th days after hemorrhage . Enzyme-linked immunosorbent assay (ELISA) was used to determine the content of sIL-6R in cerebrospinal fluid of patients in control group and intracerebral hemorrhage. MAIN OUTCOME MEASURES: The content of sIL-6R in cerebrospinal fluid of patients in control group and intracerebral hemorrhage. Results: The levels of sIL-6R in cerebrospinal fluid of the 13 controls and 32 patients with acute cerebral hemorrhage were (380.54 ± 93.05) ng / L and (1220.18 ± 878.71) ng / L, respectively.