利用粳籼杂交后代出现的不育株作细胞质雄性不育供体，先后育成了Ｋ青Ａ、Ｋ１９Ａ和Ｋ１７Ａ等籼型不育系。育成的Ｋ型杂交水稻新组合已有５个通过四川省品种审定。初步研究发现，Ｋ型胞质对提高柱头外露率和开花累积率的作用明显优于ＷＡ型胞质；在可恢性及主要数量性状上的胞质效应与ＷＡ型和Ｄ型胞质差异较小。花药发育的细胞学显示Ｋ型不育系花粉败育发生在单核后期，少量花粉发生在二核期，败育花粉以典败为主。Ｋ１７Ａ和Ｋ青Ａ花药的酯酶和过氧化物酶同工酶谱与汕Ａ存在差异。随机扩增多态性ＮＤＡ（ＲＡＰＤ）分析表明Ｋ型胞质与ＷＡ型和Ｄ型胞质的线粒体基因组存在组织结构差异。 In this study, sterile male plants derived from the indica hybrids of japonica indica were used as male sterile cytoplasm donors, and male sterile lines such as K A, K19A and K17A were bred. Five new combinations of K-type hybrid rice have been approved by Sichuan Province. Preliminary studies showed that the cytosolic effects of K type cytoplasm were significantly better than those of WA type cytoplasm on increasing the stigma exsertion rate and flowering accumulation rate. The cytosolic effects on recoverability and major quantitative traits were different from those of WA and D type cytoplasm small. Cytology of anther development showed that the pollen abortion of K-type CMS occurred in the late stage of mononuclear stage. A small amount of pollen occurred in the dinucleate stage. Esterase and peroxidase isozymes of K17A and KA anthers were different from those of Shan A. Random amplified polymorphic DNA (RAPD) analysis showed that the mitochondrial genomes between K type cytoplasm and WA type and D type cytoplasm have the same histological structure.