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目的:本研究旨在对比观察全身垂直振动、跑台运动、金雀异黄酮和氯化锂等不同干预疗法对去卵巢骨质疏松大鼠子宫雌激素受体α(ERα)和雌激素受体相关受体α(ERRα)蛋白表达的影响。方法:将80只3月龄雌性SD大鼠按体重分层后随机分为假手术组和去卵巢组。去卵巢10周时,将去卵巢组大鼠按体重分层后又随机分为去卵巢组、振动组、跑台组、金雀异黄酮组、氯化锂组和雌激素组,并开始进行不同的干预处理。干预处理8周时,腹主动脉取血处死各组大鼠,用放射免疫方法检测血清E_2水平,用Western blot检测子宫ERα和ERRα蛋白表达水平的变化。结果:大鼠去卵巢后,血清E_2水平显著下降,经雌激素处理后,血清E_2水平显著回升,但经其他几种方法处理后均无显著变化。Western blot结果显示,大鼠去卵巢后,子宫ERα蛋白表达水平显著增加,经雌激素、跑台运动、全身垂直振动和氯化锂处理后,子宫ERα蛋白表达水平显著下降,而经金雀异黄酮处理后,子宫ERα蛋白表达水平无显著变化。大鼠去卵巢后,子宫ERRα蛋白表达水平显著下降,经雌激素和金雀异黄酮干预后,子宫ERR-α表达水平显著增加,而经跑台运动、全身振动和氯化锂干预后,子宫ERR-α表达水平却显著下降。结论:跑台运动和全身垂直振动能抑制去卵巢骨质疏松大鼠子宫ERα和ERRα蛋白的表达,氯化锂能抑制ERα蛋白的表达,但促进ERRα蛋白的表达,金雀异黄酮能促进ERRα蛋白的表达,但对ERα蛋白的表达没有影响。
OBJECTIVE: The purpose of this study was to compare the effect of different interventions such as vertical body vibration, treadmill exercise, genistein and lithium chloride on the expression of estrogen receptor alpha (ERα) and estrogen receptor Effect of ERRα on the Expression of. Methods: Eighty three-month-old female Sprague-Dawley rats were randomly divided into sham operation group and ovariectomized group according to body weight. After ovariectomized for 10 weeks, the ovariectomized rats were randomly divided into ovariectomized group, sham group, treadmill group, genistein group, lithium chloride group and estrogen group according to body weight. Different interventions. At 8 weeks after intervention, rats in each group were sacrificed by abdominal aorta, serum E_2 levels were measured by radioimmunoassay, and the changes of ERα and ERRα protein expression were detected by Western blot. Results: After ovariectomized, the level of serum E_2 decreased significantly. After estrogen treatment, the level of serum E2 increased significantly, but no significant change was observed after treatment with other methods. Western blot results showed that the expression of ERαprotein in uterus was significantly increased after ovariectomy in rats, and the expression of ERαprotein in uterus decreased significantly after estrogen, treadmill exercise, vertical vibration of the whole body and lithium chloride treatment, After the flavone treatment, there was no significant change in uterine ERα protein expression. After ovariectomized, the expression of ERRαprotein in the uterus decreased significantly. After intervention with estrogen and genistein, the expression of ERR-αin the uterus increased significantly. After treadmill exercise, systemic vibration and lithium chloride intervention, ERR-α expression decreased significantly. Conclusion: Treadmill exercise and whole-body vertical vibration can inhibit the expression of ERα and ERRα protein in uterus of ovariectomized rats. Lithium chloride can inhibit the expression of ERα protein but promote the expression of ERRα protein. Genistein can promote ERRα Protein expression, but did not affect the expression of ERα protein.