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利用滴涂法和电化学聚合法层层组装了聚L-半胱氨酸/乙炔黑修饰玻碳电极,用电化学阻抗对电极进行了电化学表征。用该电极作为多巴胺的电化学传感器,循环伏安法表征显示,多巴胺的电化学信号显著增强。在优化条件下,用差分脉冲伏安法对多巴胺进行了定量分析。结果表明,多巴胺在1.67~167μmol/L内与氧化峰电流有着良好的线性关系,线性方程为:I=-0.273c-7.13μA、相关系数为0.998;检出限为0.8μmol/L。用于盐酸多巴胺注射液试样中多巴胺的分析,回收率为96.7%~103%。
Poly (L-cysteine / acetylene black) modified glassy carbon electrode was assembled by drop-coating method and electrochemical polymerization method. Electrochemical impedance was used to characterize the electrode. Using this electrode as an electrochemical sensor for dopamine, cyclic voltammetry showed that the electrochemical signal of dopamine was significantly enhanced. Under optimized conditions, dopamine was quantified by differential pulse voltammetry. The results showed that dopamine had a good linear relationship with the oxidation peak current in the range of 1.67 ~ 167μmol / L, the linear equation was: I = -0.273c-7.13μA, the correlation coefficient was 0.998; the detection limit was 0.8μmol / L. Dopamine hydrochloride for the injection of dopamine samples, the recovery was 96.7% ~ 103%.