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本文研究了高效液相色谱法测定红豆杉Taxus chinensis中紫杉醇的含量,以倍他米松作内标,在填充以YWG 80(10μm)固定相的不锈钢色谱柱上,以CH_2Cl_2-MeOH(95:5)作流动相,在UV 228 nm波长进行检测,其变异系数小于2%。
This paper studied the determination of paclitaxel in Taxus chinensis by high performance liquid chromatography with betamethasone as internal standard on stainless steel column filled with YWG 80 (10μm) stationary phase with CH 2 Cl 2 -MeOH (95:5). The mobile phase was detected at a wavelength of 228 nm with a coefficient of variation of less than 2%.