论文部分内容阅读
运用 PCR-RFLP 技术分析凡纳对虾两个引进亲本群体及其各自子一代的遗传变异情况。用一对引物对这四个群体进行 mt DNA-细胞色素氧化酶I部分片段的 PCR 扩增,扩增结果为一条约 1.25 kb的条带。用 19种限制性内切酶对该扩增条带进行酶切分析,其中 Hinf I, Sty I, Taq I 这 种酶有酶切位点。用这3种 3酶进行限制性片段长度多态性 ( RFLP )分析,共获得 种限制性类型 基因型 。而且内切酶 2 ( ) Sty I在两个不同的引进亲本群体及其各自的子一代的扩增产物的酶切中,表现出明显的差异。
PCR-RFLP was used to analyze the genetic variation of two introduced parents and their own offspring. A pair of primers were used to amplify the mt DNA-cytochrome oxidase I fragment by a pair of primers. The amplified result was a 1.25 kb band. The amplified bands were digested with 19 restriction enzymes, Hinf I, Sty I and Taq I. Restriction fragment length polymorphism (RFLP) analysis was performed using these three 3 enzymes to obtain a restriction genotype. Moreover, digestion of Sty I showed significant differences in the digestion of the amplification products of two different introduced parental populations and their respective sub-generations.