以金针菇和杏鲍菇菇脚为试材,采用单因素试验与正交实验优化酶解条件,对可溶性固形物进行提取,并研究酶解物的成分及抗氧化活性,以期为酶解产物的开发应用提供参考依据。结果表明:最佳酶解条件为食用菌水解酶1.5%、风味酶1.5%、料水比1∶30g·mL~(-1);金针菇和杏鲍菇菇脚的酶解物分别含粗蛋白24.35%和12.30%,粗多糖35.92%和37.40%,黄酮0.77、0.64mg·kg~(-1),维生素C 4.82、4.18mg·kg~(-1);金针菇和杏鲍菇菇脚酶解物均具有显著的抗氧化能力。金针菇酶解物在0.1g·L~(-1)低浓度时可明显提高秀丽线虫的抗氧化能力,最长存活时间为12d,远长于对照组(5d);杏鲍菇酶解物的抗氧化能力与浓度呈正相关,随着浓度的增加,秀丽线虫最长寿命及平均寿命均相应延长。在高浓度2.0g·L~(-1)时,秀丽线虫的最长寿命达16d,平均寿命为8.27d。 Taking Flammulina velutipes and Pleurotus ostreatus as test material, single factor experiments and orthogonal experiments were used to optimize the conditions for enzymatic hydrolysis. Soluble solids were extracted and the composition and antioxidant activity of the hydrolyzate were studied. Development and application of reference. The results showed that the best enzymolysis conditions were 1.5% of edible fungi hydrolase, 1.5% of flavor enzyme and 1:30 g · mL -1 of feed water. Enzymatic hydrolyzate of Flammulina velutipes and Pleurotus eryngii mushrooms contained crude protein 24.35% and 12.30%, 35.92% and 37.40% of crude polysaccharides, flavonoids 0.77 and 0.64 mg · kg -1, vitamin C 4.82 and 4.18 mg · kg -1, All have significant antioxidant capacity. Flammulina velutipes hydrolyzate could significantly increase the antioxidant capacity of C. elegans at low concentration of 0.1 g · L -1, the longest survival time was 12 days, which was much longer than that of the control group (5 days) Oxidation capacity and concentration were positively correlated, with the increase of concentration, the longest life expectancy of C. elegans and the average life expectancy were extended accordingly. At the highest concentration of 2.0 g · L -1, the longest life expectancy of C. elegans reached 16 days, with an average life expectancy of 8.27 days.