论文部分内容阅读
植物病原细菌通过III型分泌系统将大量的效应蛋白分泌到宿主细胞内,从而抑制宿主的先天免疫。效应蛋白Avr Xcc E1广泛存在于黄单胞菌中,然而关于Avr Xcc E1具体的作用机理尚不清楚。本研究利用野油菜黄单胞菌野油菜致病变种(Xanthomonas campestris pv.campestris,Xcc)和拟南芥互作模式系统,发现来自Xcc8004菌株的效应蛋白Avr Xcc E1对于Xcc8004在拟南芥上的全毒性是必须的,并且Avr Xcc E1可以抑制病原细菌鞭毛蛋白Flg22诱导的FRK1(Flg22-induced receptor-like kinase)基因表达,但并不抑制Flg22诱导的M AP激酶活性。通过Avr Xcc E1与GFP蛋白的融合表达,证实效应蛋白Avr Xcc E1定位在细胞膜上,并且细胞膜定位对于Avr Xcc E1在拟南芥上发挥毒性功能和在大白菜品种“中白83”上发挥无毒功能是必须的。
Phytopathogens act through the type III secretion system to secrete large quantities of effector proteins into host cells, thereby inhibiting the host’s innate immunity. The effector protein Avr Xcc E1 is widely present in X. aeruginosa, however the exact mechanism of action on Avr Xcc E1 is unclear. In this study, Xanthomonas campestris pv. Campestris (Xcc) and Arabidopsis thaliana interacting system were used to find that the effector protein Avr Xcc E1 from Xcc8004 was expressed in Arabidopsis All virulence is required and Avr Xcc E1 inhibits the Flg22-induced receptor-like kinase (FRK1) gene expression induced by the pathogenic bacterial flagellin, but does not inhibit Flg22-induced M-AP kinase activity. Confirmation of the effector protein Avr Xcc E1 on the cell membrane by fusion expression of Avr Xcc E1 with GFP protein and localization of the cell membrane to Avr Xcc E1 exert toxic functions on Arabidopsis and on Chinese cabbage variety White 83 Non-toxic function is necessary.