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近年来落叶型症状的棉花黄萎病对棉花生产的危害越来越大,为明确落叶型棉花黄萎病菌的分化特点,采用特异性分子标记方法,对我国10个主要植棉省区30个落叶型棉花黄萎病菌的培养特性和致病力进行了比较。落叶型黄萎病菌系在我国主要植棉省区已普遍存在,该菌系以菌核型为主,其微菌核呈放射状或环状。63.3%菌系的菌丝发达致密,其余菌系的菌丝较为疏松。落叶型黄萎病菌系之间的产孢量和致病力差异较大,产孢量变幅为3.7×107～18.8×107个孢子/mL;71.9%为强致病力菌系,25.0%为中等致病力。研究表明,不同植棉省区间落叶型黄萎病菌系的产孢量和致病力具有一定差异。
In recent years, the deciduous symptom of cotton Verticillium wilt is more and more damaging to the cotton production. To clarify the differentiation characteristics of the deciduous cotton Verticillium dahliae, using the specific molecular markers method, The culture characteristics and pathogenicity of deciduous cotton Verticillium dahliae were compared. Verticillium dahliae strains in China’s major cotton-growing provinces have been widespread, the bacteria to the main karyotype, the micro-bacteria were radial or ring-shaped. 63.3% strains of mycelium developed dense, other mycelial hyphae more loose. The sporulation and pathogenicity of the isolates of Verticillium dahliae varied greatly. The variation of sporulation ranged from 3.7 × 107 to 18.8 × 107 spores / mL and 71.9% to strong virulence strains, 25.0% Medium pathogenicity. The results showed that there were some differences in the sporulation and pathogenicity of the defoliating Verticillium dahliae in different cotton-growing provinces.