本文报道利用肌营养蛋白基因内缺失高发区的９对引物进行一步到位多重ＰＣＲ和变性聚丙烯酰胺凝胶电泳及银染方法对ＤＭＤ家庭进行父、母、子、胎儿的基因分析和产前诊断，结果在一块凝胶板上判读。本方法快速、准确。利用本方法并配合ＰＣＲ检测性别，对１７例家庭进行了基因分析和产前诊断。７例已出生正常婴儿，３例孕中，３例流产，４例未孕。随访结果与诊断结果相符。 This paper reports the use of nine pairs of primers for the deletion of the high-incidence region of the muscular protein genes in one-step multiplex PCR and denaturing polyacrylamide gel electrophoresis and silver staining for gene analysis and prenatal diagnosis of the DMD family parents, fetuses, fetuses Results were interpreted on a gel plate. This method is fast and accurate. Using this method and testing for genders by PCR, 17 patients were genetically analyzed and prenatal diagnosed. Seven infants were born, three were pregnant, three were aborted and four were non-pregnant. Follow-up results and diagnostic results.