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目的 研究三角帆蚌多糖的抗肿瘤活性。方法 给荷HepA腹水瘤小鼠胃饲三角帆蚌多糖 (每日剂量 5 0 ,2 5 0和5 0 0mg/kg) 8d ,检测抑瘤率、胸腺指数和脾指数 ;采用3H TdR掺入法 ,检测三角帆蚌多糖对HepA腹水瘤细胞DNA合成的影响。结果 在上述三种试验剂量 ,三角帆蚌多糖对小鼠HepA腹水瘤的抑制率分别为 3 4.9%、48.6%和 5 1.0 % ;与对照组比较 ,试验组的胸腺指数分别提高 5 5 .6% ,61.1%和 77.8% ,脾指数分别提高 8.2 % ,12 .3 %和 16.3 % ;在离体细胞培养试验中仅高浓度组 (10 0 0 μg/mL)三角帆蚌多糖对HepA瘤细胞DNA合成和细胞增殖有显著的抑制作用 (P <0 .0 5 )。 结论 三角帆蚌多糖有一定的抗肿瘤活性 ,其作用机制可能与增强胸腺免疫功能有关。
Objective To study the antitumor activity of polysaccharide from Hyriopsis Cumingii. Methods The HepA-ascitic tumor mice were fed with H. tabaci polysaccharide (daily doses of 50, 250 and 500 mg / kg) for 8 days, and the tumor inhibition rate, thymus index and spleen index were detected. 3H-TdR incorporation To detect the effect of Hyriopsis cumingii polysaccharides on DNA synthesis in HepA ascites tumor cells. Results The inhibitory rates of Hyriopsis cumingii polysaccharide on mouse HepA ascites tumor were 34.9%, 48.6% and 51.0% at the above three experimental doses, respectively; compared with the control group, the thymus index of the experimental group increased by 55.6 %, 61.1%, and 77.8%, respectively. The spleen index increased by 8.2%, 12.3% and 16.3% respectively. In the in vitro cell culture experiment, only high concentration group (100 μg / mL) DNA synthesis and cell proliferation were significantly inhibited (P <0. 05). Conclusion The polysaccharide of Hyriopsis cumingii has certain anti-tumor activity, and its mechanism may be related to the enhancement of immune function of thymus.