【摘 要】
：
As a member of pattem-recognition receptors (PRRs), the Toll-like receptor (TLR) and its signaling pathway play pivotal roles in recognizing various pathoge
【机 构】
：
TheKeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences,7NanhaiRd
【出 处】
：
18th International Pectinid Workshop(第18届国际扇贝研讨会)
论文部分内容阅读
As a member of pattem-recognition receptors (PRRs), the Toll-like receptor (TLR) and its signaling pathway play pivotal roles in recognizing various pathogen-associated molecular patterns (PAMPs), and buildup the front-line against invading pathogens.In the present study, the sequence features and mRNA expression profiles of five key genes involved in TLR signal pathway were characterized, and their functions in the immune responses were also investigated in order to validate the TLR signaling pathway and its potential roles in the immune defense of Zhikong scallop Chlamys farreri.These five genes, including CfTLR, CfMyD88, CfTRAF6, Cflκ B and CfNκB, exhibited significant similarity with their homologues from other model organisms, and contained the typical motifs.A strong interaction between the TIR domain from CfTLR and CfMyD88 protein was revealed via ELISA assays.The mRNA transcripts of these five genes were all up-regulated after LPS stimulation (Fig.1), indicating that they were involved in the immune response against LPS.
其他文献
Although the dry transport of scallop is the preferred method for the shipment of live shellfish, transport in seawater requires larger volumes therefore in
Glutathione peroxidase (GPx) is an antioxidant enzyme that protects cells from oxidative damage in the innate immune responses against bacterial infections.
Biomass reference points for fisheries management can be set using biomass estimates from a time series of scientific surveys.However, these surveys may inc
In recent years, cultrued Scallop (Patinopecten yessoensis) died on a large-scale in seas near Changhai Xian.The abscess on adductor muscle is the main symp
The cholinergic system plays an important role in the immune response.Acetylcholinesterase (AChE) is a major component of the cholinergic system, which can
C-type lectins are a superfamily of diverse proteins with one or more carbohydrate-recognition domains (CRDs) of~130 amino acid residues.They recognize and
Complement system, as the important part of innate immunity and one of the major effecter arms of immune responses, play a critical role in innate immune de
Catecholamine system is a crucial neuroendocrine system, responsible for not only the transmission of neural signal but also the regional modulator of immun
Two experiments were conducted to investigate the immune responses and cellular energy allocation (CEA) of Zhikong scallop Chlamys farreri under the acute V