RT-PCR分析相关论文
以17-α甲基睾丸酮投喂赤点石斑鱼（Epinephelus akaara），成功地获得了性逆转的有功能的雄鱼。用SMART cDNA合成和长片段PCR技术构建了......
设计简并引物克隆了PPO基因cDNA片段,并根据获得的序列信息用cDNA末端快速扩增(RACE)方法从双孢蘑菇中克隆到了两个PPO同源基因的c......
目的： 目前，肺癌已成为全球最常见的癌症，同时也是最主要的癌症致死疾病。在肺癌中，非小细胞肺癌(NSCLC)的占比达到了80-85%。微小......
Neomycin biosynthesis is regulated positively by AfsA-g and NeoR in Streptomyces fradiae CGMCC 4.738
Neomycins are a group of aminoglycoside antibiotics with both clinical and agricultural applications.To elucidate the re......
Pod1 is a member of the basic helix-loop-helix(bHLH) family of transcription factors that have been implicated in the re......
Rad51/RadA paralogs found in eukaryotes and euryarchaea play important roles during recombination and repair,and mutatio......
水稻白叶枯病是水稻生产中最严重病害之一。前期鉴定发掘的水稻资源材料Y238对白叶枯病具有广谱抗性,其衍生的以IR24为遗传背景的......
本研究利用RACE技术在中国对虾血细胞内克隆到一个整合素β亚基(β-integrin)cDNA 全序列,序列全长2853 bp,含一个2349 bp的开......
石栗(Aleurites moluccana)是大戟科(Euphorbiaceae)的木本油料植物,其种子油脂含量很高,是一种很重要的生物质能源植物。本试验克......
黄花苜蓿(Medicago falcalta L.)是耐寒性很强的豆科牧草，为了探讨其耐低温的可能机制，根据本实验室庞朝枢构建的“黄花苜蓿冷诱导基......
板桥党参系桔梗科植物川党参Codonopsis tangshen Oliv.的干燥根,因产于湖北恩施板桥镇而得名,是益气健脾的上乘良药。已知它富含......
OsGL1(Oryza sativa L.Glossy1)是一类植物中存在的具有保守结构域的基因，其表达产物属于脂肪酸羟化酶超家族中的固醇去饱和酶，家族......
黄孢原毛平革菌(Phanerochaetechrysosporium)属于白腐担子真菌，是研究木质素生物降解的模式生物，该菌的基因组序列已由美国联合基因......
叶色突变体是研究光合色素合成，叶绿体发育调控和光合作用机制的重要材料。目前的研究结果表明，水稻叶色相关基因在12条染色体上均有......
为了明确糯质和非糯谷子Waxy基因c DNA序列变异,为品质育种提供实验依据,以2份糯质和2份非糯谷子为材料,利用特异RT-PCR和序列比对......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
瞄准：调查 hexahydrocurcumin (HHC ) 的能力在由在 cyclooxygenase (艇长) 上集中 -2 表示禁止 HT-29 细胞的生长提高 5 氟尿嘧啶(......
The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an im......
Aminoacyl-tRNA syntheses(AARS) can catalyze the adenosine triphosphate(ATP)-dependent acylation of their cognate tRNA(s)......
利用同源克隆方法，在芥菜型油菜中克隆了DFR基因。在DNA和cDNA中扩增的DFR基因大小分别为1612bp和1214bp。该基因含有5个内含子，开放......
对来自新疆不同地区的12个甜菜坏死黄脉病毒(BNYVV)分离物的核酸组分进行RT-PCR分析表明,不同地区的BNYVV分离物RNA组分有一定差异......
Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene （ZmALDH7B6） in R
Aldehyde dehydrogenases(ALDHs) represent a large protein family, which includes several members that catalyze the oxidat......
对卵形鲳鲹（Trachinotus ovatus）腹腔注射20、50、100 mg/kg 的激动剂非诺贝特,用RT-PCR 检测肝脏、肌肉中PPAR α 和CPTI 基因表达......
瞄准：评估 mitochondrial 肿瘤坏死因素的效果在中国 colorectal 癌症(CRC ) 的淋巴节点转移(LNM ) 上的联系受体的 protein-1 (TRA......
瞄准：调查在人的乳突淋瘤之间的协会病毒(HPV ) 感染和 colorectal 癌症。方法：有包括 6 stageI， 24 阶段 II， 21 阶段 III，和 18 阶段 ......
瞄准：在 colorectal 癌症和息肉组织样品屏蔽微分表示基因。方法：包含 colorectal 腺癌和 colorectal 息肉对正常 mucosae 的 16 个......
瞄准：在 apoptosis 和房间周期拘捕在人的胃的癌症和它的机制调查 15-hydroxyprostaglandin 脱氢酶(15-PGDH ) 的表示。方法：15-PGDH......
Virus-induced gene silencing(VIGS) offers a rapid and high throughout technology platform for the analysis of gene funct......
The production of transgenic sweetpotato (cv.Xushu 18) plants exhibiting enhanced salt tolerance using salt overly sensi......
Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene （Ha-acp1） from Het
For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands c......
Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichod
A endochitinase gene(Tch) from the fungus Trichoderma viride was introduced into broccoli(Brassica oleracea var. italica......
Overexpression of a modified AM79 aroA gene in transgenic maize confers high tolerance to glyphosate
It has previously been shown that a bacterial 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS) encoding gene AM79 aroA......
Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice （Oryza rufi
The use of cytoplasm male sterility(CMS) is crucial for three-line hybrid seed production. Two types of CMS have been di......
Using the Phosphomannose Isomerase （PMI） Gene from Saccharomyces cerevisiae for Selection in Rice Tr
The phosphomannose isomerase (PMI) gene from Saccharomyces cerevisiae acted as selectable marker and mannose acted as se......
文章根据已知植物eEF-1a（编码 Eukaryotic elongation factor1-alpha）基因的保守序列设计引物，采用 RT- PCR的方法扩增独行菜（Lepidium......
根据MADS-box基因保守区结构，设计简并引物，从板栗（Castanea mollissima）中分离出花特异表达基因的cDNA片段。并通过5’RACE方法获得了......
从微小牛蜱克隆到1个新的铁蛋白编码基因,cDNA全长642bp,编码区为123-639bp,编码172个氨基酸残基,该蛋白预测的分子量为19.9ku,等......
High-tillering dwarf mutant gsor23 was generated from an indica rice variety Indica9 radiatied byγ-ray.Genetic analysis......
报道经过农杆菌介导将人降钙素基因相关肽(calcitonin gene-related peptide,CGRP)基因由马铃薯块茎专一表达class I patatin基因5......
DEAD—box RNA解旋酶是一种特殊的RNA分子伴侣，参与了RNA代谢，包括前体RNA剪接、核糖体合成、RNA降解以及基因表达，并对植物的发育和......
利用RT-PCR方法分析了生长于冷杉木片上的黄孢原毛平革菌木质素过氧化物酶基因lipA2(GLG3)、lipC1(GLG2)、lipC2(GLG5)、lipD2(GLG......
Object: To identify transcript variants and expression patterns of porcine Mitf.Materials and methods: A pairwise BLAST ......
Heterologous Expression of Isopentenyl Transferase （IPT） Gene Directed by Senescence Associated Rece
[Objective] This study aimed to investigate the expression of IPT gene directed by GmSARK promoter in Arabidopsis.[Metho......
Eukaryotic translation initiation factor 5A( eIF5A) is a protein-translation initiation factor in eukaryotic cells. Rece......
The growth and development of rice are closely related with temperature. In order to clarify the mechanism of high tempe......
Identification of normalization factors for quantitative real- time RT-PCR analysis of gene expressi
量的即时反向的抄写聚合酶链反应(qRT-PCR ) 广泛地在基因表示的研究被使用。在大多数这些研究，没有确认，家务基因被用作内部参考书......
Ebola 病毒(EBOV ) 和 Marburg 病毒(MARV ) 是有在人和非人类的首领的高死亡率的严重出血性的发烧的原因的代理人，当前没有准许的......
