目的研究三九膏的体外透皮吸收规律。方法通过Franz扩散池获得接收液,采用HPLC法测定芥子碱硫氰酸盐,计算三九膏的体外透皮吸收的累积渗透率,探究其拟合模型。结果三九膏中芥子碱硫氰酸盐12 h的累积渗透率在22%左右,体外透皮吸收规律符合希古契模型。结论三九膏中芥子碱硫氰酸盐累积透过量在时间上与临床治疗贴敷时间吻合,为三九膏临床治疗确定最适宜贴治时间提供了依据。 Objective To study the law of skin penetration of Sanjiu ointment in vitro. Methods The receiving liquid was obtained by Franz diffusion cell. The erucic acid thiocyanate was determined by HPLC. The cumulative permeability of Sancuxiang ointment in vitro was calculated and the fitted model was explored. Results The accumulative permeation rate of mustard alkali thiocyanate 12% in Sanjiu cream was about 22%. The transdermal absorption in vitro was in accordance with the Greek ancient style. Conclusions The accumulative permeation amount of thiocyanate in Sanjiu cream agrees with the clinical application time in time, which provides the basis for determining the most suitable sticking time for the clinical treatment of Sanjiu cream.