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采用聚合酶链反应(PCR)技术.并配合限制性片段长度多态性(RFLP)分析。对41例大肠癌组织APC/MCC和DCC基因的杂合缺失(LOH)进行研究。APC基因LOH率为28.0％(7/25),MCC基因LOH率为36.40％(8/22),两者综合分析LOH率为38.9％(14/38)。DCC基因LOH率为55.3％(21/38)。DCC基因在有淋巴结转移组的LOH率(80.0％),显著高于无淋巴结转移组(39.1％)(P<0.05),在Dukes C、D期组的LOH率(71.4％)显著高于A、B期组(35.3％)。以上结果提示,APC/MCC和DCC基因的LOH是大肠癌常见的基因改变,DCC基因LOH的测定有可能成为大肠癌病人预后评估的指标。
Polymerase chain reaction (PCR) technique was used, combined with restriction fragment length polymorphism (RFLP) analysis. The heterozygous deletion (LOH) of APC/MCC and DCC genes in 41 cases of colorectal cancer was studied. The LOH rate of APC gene was 28.0% (7/25), and the LOH rate of MCC gene was 36.40% (8/22). The overall analysis of the LOH rate was 38.9% (14/38). The LOH rate of DCC was 55.3% (21/38). The LOH rate of the DCC gene in the lymph node metastasis group (80.0%) was significantly higher than that in the no lymph node metastasis group (39.1%) (P<0.05), and the LOH rate in the Dukes C and D stage groups (71.4%) was significantly higher than that in the A group. Group B (35.3%). The above results suggest that LOH of APC/MCC and DCC genes is a common genetic change in colorectal cancer, and the determination of DCH gene LOH may serve as an indicator of prognosis in colorectal cancer patients.